Keratinase H328 and Keratinase H328-Proteinase K Treatments for Wool Surface
Keratinase H328 and Keratinase H328-Proteinase K Treatments for Wool Surface
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DOI：
10.2115/fiberst.2017-0018
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发表时间：
2017-06
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通讯作者：
Chendi Tu;F. Kawai;Kunihiko Watanabe;Kiyomi Okada;S. Sukigara
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作者：
Chendi Tu;F. Kawai;Kunihiko Watanabe;Kiyomi Okada;S. Sukigara
对羊毛角质层进行环保改性以提高羊毛织物的舒适性是该行业的一个重要目标。本文研究了一种来源于红色小栖热菌（Meiothermus ruber）H328的角蛋白酶（Keratinase H328）对羊毛纤维角蛋白的作用。角蛋白酶H328处理对羊毛鳞片有腐蚀作用。该效果的特点是使用扫描电子显微镜（SEM），氨基酸分析和傅立叶变换红外衰减全反射（FTIR-ATR）的物理化学分析。随后，将角蛋白酶H328处理与蛋白酶K处理组合用于羊毛织物。在80%和90%相对湿度下，酶处理后的羊毛织物的回潮率均高于对照。角蛋白酶-蛋白酶处理降低了最大拉伸应变和最大应力，而不显著改变杨氏模量。（接收日期：2017年3月8日;接受日期：2017年4月16日）#通讯作者：电子邮件：sukigara@kit.ac.jp角蛋白酶H328和角蛋白酶H328-羊毛表面的蛋白酶K处理Chendi Tu，Fusako Kawai，Kunihiko Watanabe，Kiyomi Okada，and Sachiko Sukigara * 1京都工业大学高级纤维科学系，松崎，Sakyo-ku，京都606-8585，日本 * 2京都工业大学纤维和纺织科学中心，松崎，Sakyo-ku，京都606-8585，日本 * 3京都府立大学应用生命科学部，Shimogamo，Sakyo-ku，京都606-8522，日本 * 4京都大学化学工程系，京都大学Katamoto校区，Nishikyo-ku，京都615-8510，日本J. Fiber Sci.技术人员：73（6），126-134（2017）doi 10.2115/fiberst.2017-0018 © 2017 The Society of Fiber Science and Technology，Japan 126 Journal of Fiber Science and Technology（JFST），Vol.73，No.6（2017）我们使用来自Meiothermus ruber H328的部分纯化的角蛋白酶（角蛋白酶H328）来降解羊毛角蛋白。进一步研究了角蛋白酶H328和蛋白酶K对羊毛织物的联合处理，以了解角蛋白酶H328对羊毛角质层的影响。对酶处理的样品进行氨基酸分析和红外光谱分析。通过测定羊毛织物的吸湿性能和拉伸性能，评价了角蛋白酶-蛋白酶联合处理对羊毛织物的影响。
Environmentally friendly modification of the wool cuticle to enhance wool fabric comfort is an important goal for the industry. The present study describes the effect of a keratinase from Meiothermus ruber H328 (Keratinase H328) on wool fiber keratin. Keratinase H328 treatment corroded the wool scale. The effect was characterized by physicochemical analyses using scanning electron microscopy (SEM), amino acid analysis, and Fourier-transform infrared-attenuated total reflectance (FTIR-ATR). Subsequently, Keratinase H328 treatment was combined with Proteinase K treatment for the wool fabric. Enzymatically treated wool fabric showed a higher moisture regain than its control at 80% and 90% R.H. The keratinase-proteinase treatment reduced the maximum tensile strain and maximum stress without altering Young’s modulus significantly. (Received 8 March, 2017; Accepted 16 April, 2017) # corresponding author: E-mail: sukigara@kit.ac.jp Keratinase H328 and Keratinase H328-Proteinase K Treatments for Wool Surface Chendi Tu, Fusako Kawai, Kunihiko Watanabe, Kiyomi Okada, and Sachiko Sukigara *1Department of Advanced Fibro-Science, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan *2Center for Fiber and Textile Science, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan *3Division of Applied Life Sciences, Kyoto Prefectural University, Shimogamo, Sakyo-ku, Kyoto 606-8522, Japan *4Department of Chemical Engineering, Kyoto University, Kyoto University Katsura Campus, Nishikyo-ku, Kyoto 615-8510, Japan J. Fiber Sci. Technol., 73(6), 126-134 (2017) doi 10.2115/fiberst.2017-0018 © 2017 The Society of Fiber Science and Technology, Japan 126 Journal of Fiber Science and Technology (JFST), Vol.73, No. 6 (2017) We used partially purified keratinase from Meiothermus ruber H328 (Keratinase H328) to degrade wool keratin. Further combined treatment of Keratinase H328 and Proteinase K on wool fabric was examined to find the effect of Keratinase H328 on the wool cuticle. Enzyme-treated samples were subjected to amino acid analysis, and IR spectroscopy. The impact of combined keratinase-proteinase treatment on wool cuticles was evaluated by the moistureabsorbing ability and tensile properties which are related to wool’s wearing comfort.