BnaMPK6 is a determinant of quantitative disease resistance against Sclerotinia sclerotiorum in oilseed rape
BnaMPK6 is a determinant of quantitative disease resistance against Sclerotinia sclerotiorum in oilseed rape
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BnaMPK6 是油菜中对核盘菌的定量抗病性的决定因素
DOI：
10.1016/j.plantsci.2019.110362
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发表时间：
2020
期刊：
影响因子：
5.2
通讯作者：
Tan Xiao-Li
中科院分区：
文献类型：
--
作者：
Wang Zheng;Zhao Feng-Yun;Tang Min-Qiang;Chen Ting;Bao Ling-Li;Cao Jun;Li Yu-Long;Yang Yan-Hua;Zhu Ke-Ming;Liu Shengyi;Tan Xiao-Li
油菜(Brassica Napus)菌核病是一种毁灭性的病害，造成重大经济损失。B的阻力响应。纳波斯反对S。核盘菌表现出典型的数量抗病(QDR)特征，但这种QDR的分子决定因素在很大程度上是未知的。在本研究中，我们分离到了AB。并发现Bna MPK6的表达对感染有高度的响应。菌核化和水杨酸(SA)或茉莉酸(JA)处理。此外，BNAMPK6的过表达(OE)显著增强了对S的抗性。而BnaMPK6中的RNAi显著降低了这种抗性。这些结果表明，BnaMPK6在防御TOS中起着重要作用。核盘菌。此外，还研究了与SA、JA和乙烯(ET)介导的信号转导相关的防御基因在Bna MPK6-RNAi、WT和Bna MPK6-OE植株中的表达。因此，Bna MPK6激活ET信号通路可能在防御中起作用。此外，还定位了4个编码Bna MPK6的同源基因座。纳普斯基因组。通过对4个基因座的等位基因分析和表达分析，证明了BnaA03.MPK6基因座对抗菌核病的QDR有重要贡献。我们的数据表明，BnaMPK6是一个以前未知的QDR抗STS的决定因素。菌核发光菌B。油菜籽。
Sclerotinia sclerotiorumcauses a devastating disease in oilseed rape (Brassica napus), resulting in major economic losses. Resistance response ofB. napusagainstS. sclerotiorumexhibits a typical quantitative disease resistance (QDR) characteristic, but the molecular determinants of this QDR are largely unknown. In this study, we isolated aB. napusmitogen-activated protein kinase gene,BnaMPK6, and found thatBnaMPK6expression is highly responsive to infection byS. sclerotiorumand treatment with salicylic acid (SA) or jasmonic acid (JA). Moreover, overexpression (OE) ofBnaMPK6significantly enhances resistance toS. sclerotiorum, whereas RNAi inBnaMPK6significantly reduces this resistance. These results showed thatBnaMPK6plays an important role in defense toS. sclerotiorum. Furthermore, expression of defense genes associated with SA-, JA- and ethylene (ET)-mediated signaling was investigated inBnaMPK6-RNAi, WT andBnaMPK6-OE plants afterS. sclerotioruminfection, and consequently, it was indicated that the activation of ET signaling byBnaMPK6may play a role in the defense. Further, four BnaMPK6-encoding homologous loci were mapped in theB. napusgenome. Using the allele analysis and expression analysis on the four loci, we demonstrated that the locusBnaA03.MPK6makes an important contribution to QDR againstS.sclerotiorum. Our data indicated thatBnaMPK6is a previously unknown determinant of QDR againstS. sclerotioruminB. napus.