In vitro resorptive activity of isolated chick osteoclasts: effects of carbonic anhydrase inhibition.
In vitro resorptive activity of isolated chick osteoclasts: effects of carbonic anhydrase inhibition.
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分离的鸡破骨细胞的体外再吸收活性：碳酸酐酶抑制的影响。
DOI：
10.1002/jbmr.5650060111
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Gay,CV
中科院分区：
文献类型：
--
作者：
Hunter,SJ;Rosen,CJ;Gay,CV
碳酸酐酶抑制剂5-[3-羟基苯甲酰基]-2-噻吩磺酰胺在10−7M剂量下，可使鸡破骨细胞形成的吸收陷窝面积减少37%。在10−9和10−7M剂量下，HTS也能有效地减少破骨细胞的酸形成(分别为14%和36%)。此外，HTS的作用很容易通过去除该试剂而逆转，这表明它对细胞没有毒性影响。本研究表明HTS对骨吸收的抑制作用是在破骨细胞的成酸机制水平上起作用的，并支持碳酸酐酶在此过程中起中心作用的观点。
A potent inhibitor of carbonic anhydrase, 5‐[3‐hydroxybenzoyl]thiophene‐2‐sulfonamide (HTS), was shown to cause a 37% reduction in the area of resorption pits formed by isolated chick osteoclasts when used at a dose of 10−7M. HTS at doses of 10−9and 10−7M was also effective in reducing acid formation by the osteoclasts (14 and 36%, respectively). Additionally, the effect of HTS was found to be readily reversed by removing the agent, showing that it does not exert a toxic effect on the cells. This study indicates that the inhibitory effect of HTS on bone resorption is at the level of the acid‐forming mechanism in osteoclasts and supports the view that carbonic anhydrase has a central role in the process.