Evaluating the effect of soft lining materials on the growth of yeast.
Evaluating the effect of soft lining materials on the growth of yeast.
复制标题
评估软衬里材料对酵母生长的影响。
DOI：
10.1016/s0022-3913(98)70153-2
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Kalachandra,S
中科院分区：
文献类型：
--
作者：
Wright,PS;Young,KA;Riggs,PD;Parker,S;Kalachandra,S
问题陈述Soft衬里材料继续在临床可拆卸义齿中占有一席之地。目的(1)评价长期软衬材料对酵母生长的影响；(2)研究5种软衬材料对3种酵母菌生长的影响。材料与方法以白色念珠菌、热带念珠菌和等菌(原克氏念珠菌)为实验对象，研究了5种软衬材料对3种酵母生长的影响。血液琼脂平板上培养的软衬材料条对酵母菌的生长抑制作用进行了检测。软衬材料浸泡在无菌胰酶、大豆肉汤或水中，接种酵母并培养。测量酵母初始负荷3天后每毫升菌落形成单位的变化。采用独立配对学生t检验进行统计分析。结果两种软衬材料对酵母生长均有抑制作用。尽管有足够的活菌存在，但无论是试验组还是对照组，最初的酵母菌负荷与3天的结果之间的差异都很小，这表明该材料在这段时间内不支持被测试酵母的生长。结论口腔环境中与软衬材料相关的酵母菌的增加很可能与口腔中易获得的营养物质以及这些材料的维护和清洁困难有关。(J Prosthet Dent 1998；79：404-9.)
Statement of problemSoft lining materials continue to have a place in clinical removable prosthodontics. However, there is an increased probability of yeast colonization on soft lining materials.PurposeThis study (1) assessed a method of evaluating the effect of long-term soft lining materials on the growth of yeast and (2) investigated the effect five soft lining materials had on the growth of three species of yeast.Material and methodsCoe Supersoft, Novus, and three experimental soft lining materials were investigated together with Candida albicans, Candida tropicalis , and Issatchenkia oreintalis (formerly Candida krusei ) yeasts. Strips of soft lining material incubated on blood agar plates were examined for inhibition of the growth of yeast. Soft lining materials soaked in sterile trypticase soya broth or water were inoculated with yeast and incubated. The change in colony forming units per milliliter from the initial load of yeast at 3 days was measured. Statistical analysis was performed with an independent paired Student t test.ResultsInhibition of yeast growth occurred for two soft lining materials. Despite the presence of sufficient viable organisms, differences between the initial load of yeast and the 3-day results were mostly small, both for the test and control groups, suggesting that the material does not support the growth of the tested yeast during this period.ConclusionsThe often described increased prevalence of yeast associated with soft lining materials in the oral environment is likely related to readily available nutrients in the mouth and the difficulty in maintaining and cleaning these materials. (J Prosthet Dent 1998;79:404-9.)