Nitrate reductase activities in plants from different ecological and taxonomic groups grown in Japan
Nitrate reductase activities in plants from different ecological and taxonomic groups grown in Japan
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DOI：
10.1111/1440-1703.12083
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发表时间：
2020-09-01
影响因子：
2
通讯作者：
Tokuchi, Naoko
中科院分区：
文献类型：
--
作者：
Koyama, Lina A.;Terai, Masakazu;Tokuchi, Naoko
植物通常利用土壤无机氮、铵（NH 4 +-N）和硝酸盐（NO3--N）作为氮的来源，氮是一种必需的营养素。吸收后的同化过程有很大的不同，从铵到硝酸盐。硝酸盐在合成氨基酸之前必须在植物组织中还原为铵，而铵在其吸收后直接和立即合成氨基酸。硝酸还原酶是催化硝酸盐同化的第一步和限速步骤的酶，将硝酸盐还原为亚硝酸盐。它是一种底物诱导酶，诱导硝酸还原酶的能力在植物物种之间差异很大。体内硝酸还原酶活性（NRA）通常使用植物组织的细切片作为孵育期间的亚硝酸盐产生速率来测量，并且其可用作植物硝酸盐使用的指标。在这里，我们提出了在体内NRA的叶子，共108种，包括乔木，小树，灌木，草本植物，藤蔓植物和苔藓。75个物种，细根中的NRA也被确定。至少有20种采样植物是进口的，并用于科学或工业目的，但大多数采样植物原产于日本。主要在欧洲进行了几项植物硝酸盐还原酶清单研究，这些研究提供了关于欧洲植物硝酸盐利用能力的具体物种的信息。然而，据我们所知，迄今为止还没有公布的清单的NRA的植物在日本，许多特有种分布。我们的数据集包含植物NRA，包括物种，姓氏，生活型，叶寿命（万年青或落叶），生长阶段，样品采集季节，生长条件（自然或栽培）以及其他处理/条件（如适用）。本研究提供的数据可能有助于未来的工作，需要有关硝酸盐利用能力或硝酸盐偏好的植物物种特征的信息。发表在期刊数据论文部分的这篇摘要的完整数据集可以在JaLTER的MetaCat中以电子格式获得。[在首次在线发布后，于2020年9月7日添加了更正：JaLTER URL已更新。
Plants generally use soil inorganic nitrogen, ammonium (NH4+-N), and nitrate (NO3--N) as sources of nitrogen, an essential nutrient. The assimilation processes after uptake differ considerably from ammonium to nitrate. Nitrate must be reduced to ammonium in plant tissue before it is synthesized to amino acids, while ammonium is directly and immediately synthesized to amino acids after its uptake. Nitrate reductase is an enzyme that catalyzes the first and rate-limiting step of nitrate assimilation, reducing nitrate to nitrite. It is a substrate-inducible enzyme, and the capacity to induce nitrate reductase varies greatly among plant species. In vivo nitrate reductase activity (NRA) is generally measured as a nitrite production rate during incubation using fine cut pieces of plant tissue, and it is applicable as an indicator on plant nitrate use. Here we present in vivo NRA of leaves from a total of 108 species including arboreal trees, small trees, shrubs, herbs, a vine and a moss. For 75 of the species, NRA in fine roots was also determined. At least 20 species in sampled plants were imported and planted for scientific or industrial purposes, but most sampled species were native to Japan. Several inventory studies of plant NRA have been conducted, mainly in Europe, and they provided information on the species-specific capacity of nitrate use by plants in Europe. However, to our best knowledge, there has been hitherto no published inventory of the NRA of plants in Japan where many endemic species are distributed. Our dataset contains plant NRA with species, family name, life form, leaf lifespan (evergreen or deciduous), growth stage, the season of sample collection, growth conditions (natural or cultivated) and other treatments/conditions when applicable. The data provided by this study may contribute to future works that require information regarding the plant species characteristics for nitrate use capacity or nitrate preference. The complete data set for this abstract published in the Data Paper section of the journal is available in electronic format in MetaCat in JaLTER at . [Correction added on 7 September 2020, after first online publication: JaLTER URL has been updated.]