Epididymal C4b-binding protein is processed and degraded during transit through the duct and is not essential for fertility
Epididymal C4b-binding protein is processed and degraded during transit through the duct and is not essential for fertility
复制标题
附睾 C4b 结合蛋白在通过导管的过程中被加工和降解，对于生育来说不是必需的
DOI：
10.1016/j.reth.2015.11.001
复制
发表时间：
2015
期刊：
影响因子：
2.8
通讯作者：
Rick A Wetsel
中科院分区：
文献类型：
--
作者：
Mayumi I Nonaka;Eva Zsigmond;Akihiko Kudo;Hayato Kawakami;Kaoru Yoshida;Manabu Yoshida;Natsuko Kawano;Kenji Miyado;Masaru Nonaka;Rick A Wetsel
母乳的摄入有利于新生儿的生长，其影响被认为会持续到成年期。我们最近报道，饮食中磷不足会降低母乳促进小鼠婴儿生长的能力。然而，如何磷赋予这种能力的milk仍不清楚。MethodsTo解决这个问题，我们进行了生化和生理比较分泌的牛奶从C57 BL/6 J小鼠喂食低磷饮食（LPD）或正常磷控制diet.ResultsAlthough血清磷浓度下降，体重的母鼠不受影响。相比之下，婴儿体重显著降低，成年后观察到侏儒样症状。定量分析显示，血清生长激素（GH）浓度大幅降低，同时胰岛素样生长因子1和成纤维细胞生长因子23降低。免疫组化分析显示，异位脂肪堆积在婴儿小鼠的肝脏沿着血胆固醇水平升高。此外，电子显微镜显示的脆弱性的外膜的milk droplet.ConclusionsOur结果表明，磷是必不可少的乳滴，其功能作为一个刺激剂的生长因子分泌的婴儿后代的形成。
IntroductionBreast milk intake facilitates neonatal growth, and its effect is assumed to last long into the adulthood. We recently reported that dietary phosphorus insufficiency reduces the ability of breast milk to promote infant growth in mice. However, how phosphorus confers this ability to milk is still unclear.MethodsTo address this issue, we performed biochemical and physiological comparisons of milk secreted from C57BL/6J mice fed a low-phosphorus diet (LPD) or a normal-phosphorus control diet.ResultsAlthough serum phosphorus concentration was decreased, the body weight of mother mice was unaffected. By contrast, infant body weight was significantly reduced, and dwarfism-like symptoms were observed in adulthood. Quantitative analysis revealed that the serum concentration of growth hormone (GH) was substantially reduced, and concomitantly insulin-like growth factor 1 and fibroblast growth factor 23 were decreased. Immunohistochemical analysis revealed ectopic fat accumulation in the livers of infant mice along with increased blood cholesterol level. Moreover, electron microscopy indicated fragility of the outer membrane of milk droplets.ConclusionsOur results suggest that phosphorus is essential for the formation of milk droplets, which function as a stimulator of growth factor secretion in infant offspring.