LAURDAN and PRODAN as polarity-sensitive fluorescent membrane probes
LAURDAN and PRODAN as polarity-sensitive fluorescent membrane probes
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DOI：
10.1023/a:1020528716621
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发表时间：
1998-12-01
影响因子：
2.7
通讯作者：
Gratton, E
中科院分区：
文献类型：
--
作者：
Parasassi, T;Krasnowska, EK;Gratton, E
综述了2-二甲氨基-6-月桂酰基萘（LAURDAN）等萘衍生物的稳态和动态荧光光谱性质，以说明它们对环境极性的敏感性。在不同极性的溶剂和磷脂囊泡在两个相态中获得的结果。在极性溶剂中观察到的发射红移和在磷脂液晶相的基础上解释的偶极周围的荧光萘部分的这些探针的溶剂分子的弛豫。在磷脂的环境中，实验证据表明，排除分子内的二甲氨基和羰基基团在萘和整个荧光部分的重新取向的相对重新取向。在磷脂双层中观察到的LAURDAN和PRODAN的溶剂偶极弛豫归因于膜界面存在少量水分子。还提出了在D2 O和H2O中制备的磷脂囊泡中的LAURDAN发射之间的比较。文中还讨论了广义极化函数的定义和推导。
The steady-state and dynamic fluorescence spectral properties of 2-dimethylamino-6-lauroylnaphthalene (LAURDAN) and several other naphthalene derivatives are summarized to illustrate their sensitivity to the polarity of the environment. Results obtained both in solvents of different polarity and in phospholipid vesicles in two phase states are presented. The emission red shift observed in polar solvents and in the phospholipid liquid-crystalline phase is explained on the basis of dipolar relaxation of solvent molecules surrounding the fluorescent naphthalene moiety of these probes. In phospholipid environments, experimental evidence is shown that excludes the intramolecular relative reorientation of the dimethylamino and carbonyl groups in the naphthalene and the reorientation of the entire fluorescent moiety. The solvent dipolar relaxation observed for LAURDAN and PRODAN in phospholipid bilayers has been attributed to a small number of water molecules present at the membrane interface. A comparison between LAURDAN emission in phospholipid vesicles prepared in D2O and in H2O is also presented. The definition and the derivation of the generalized polarization function are also discussed.