Serum metabolomics study of nutrient metabolic variations in chronic heat-stressed broilers
Serum metabolomics study of nutrient metabolic variations in chronic heat-stressed broilers
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DOI：
10.1017/s0007114518000247
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发表时间：
2018-04-14
影响因子：
3.6
通讯作者：
Gao, Feng
中科院分区：
文献类型：
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作者：
Lu, Zhuang;He, Xiaofang;Gao, Feng
为研究热应激对肉仔鸡代谢的影响，将144只肉仔鸡随机分为正常对照组（NC）、热应激组（HS）和配对饲养组（PF），采用生长性能、胴体性状、生化指标和代谢组学技术对热应激对肉仔鸡代谢的影响进行了监测。泄殖腔温度的上调证实了我们的实验成功地诱导了慢性热应激。热应激组的日增重和日采食量显著低于对照组，分别降低28.76%和18.42%（P < 0.001），料重比显著高于对照组，提高14.49%（P=0.003）;热应激使腿重比（P=0.027）和肌内脂肪比（P < 0.001）增加，胸重比（P=0.009）降低。当比较HS和NC组以及HS和PF组时，我们的代谢组学方法分别鉴定出78种和34种代谢物，具有显著不同的水平（投影值的变量重要性> 1且P < 0.05）。HS组的更大的料重比与腿，腹部脂肪，皮下脂肪和肌内脂肪的比例和水平的一些游离氨基酸（脯氨酸，L-半胱氨酸，蛋氨酸和苏氨酸）显着正相关，但与乳房的比例和水平的一些NEFA（硬脂酸，花生四烯酸，棕榈酸和油酸）呈负相关。这些结果表明，热应激肉鸡处于负能量平衡，不能有效地动员脂肪，从而导致蛋白质分解，从而影响生长性能和胴体特性。
To investigate the effects of heat stress on broiler metabolism, we assigned 144 broilers to normal control (NC), heat stress (HS) or pair-fed (PF) groups and then monitored the effects using growth performance, carcass characteristics, biochemical assays and GC-MS-based metabolomics. The up-regulation of cloacal temperature confirmed that our experiment was successful in inducing chronic heat stress. The average daily gain and average daily feed intake of the HS group were significantly lower than those of the NC group, by 28.76 and 18.42%, respectively (P < 0.001), whereas the feed:gain ratio was significantly higher, by 14.49% (P=0.003), and heat stress also increased leg proportion (P=0.027) and intramuscular fat proportion (P < 0.001) and decreased breast proportion (P=0.009). When comparing the HS and NC groups and HS and PF groups, our metabolomics approach identified seventy-eight and thirty-four metabolites, respectively, with significantly different levels (variable importance in the projection values > 1 and P < 0.05). The greater feed:gain ratio of the HS group was significantly positively correlated with the leg, abdominal fat, subcutaneous fat and intramuscular fat proportions and levels of some free amino acids (proline, L-cysteine, methionine and threonine) but was negatively correlated with breast proportion and levels of some NEFA (stearic acid, arachidonic acid, palmitic acid and oleic acid). These findings indicated that the heat-stressed broilers were in negative energy balance and unable to effectively mobilise fat, thereby resulting in protein decomposition, which subsequently affected growth performance and carcass characteristics.