Diurnal variation in the glycogen content of the human liver using 13C MRS
Diurnal variation in the glycogen content of the human liver using 13C MRS
复制标题
DOI：
10.1002/nbm.4289
复制
发表时间：
2020-06-01
影响因子：
2.9
通讯作者：
Takahashi, Hideyuki
中科院分区：
文献类型：
--
作者：
Iwayama, Kaito;Onishi, Takahiro;Takahashi, Hideyuki
组织中的糖原不仅作为碳水化合物储备，而且还作为分子传感器，能够激活信号通路以响应身体活动。虽然骨骼肌中的糖原主要是局部能量底物，但肝脏中的糖原作为葡萄糖储备，即使在睡眠状态下也能维持体内正常的血糖水平。本研究的目的是比较正常情况下受试者肌肉和肝脏中糖原的日变化。使用非侵入性技术C-13MRS对10名年轻、健康、男性志愿者的肌肉和肝脏中糖原含量进行了测量。受试者仍然久坐不动，每天测量六次糖原浓度。提供实验餐以实现个体能量平衡，根据日本患者的能量需求指南进行估计。与晚餐后1h(第1天20：00)相比，肌糖原变化最大的是3.1+/-8.2 mmol/L(第2天16：00)。而肝脏中糖原含量在隔夜禁食期间(第二天07：00)下降了80.6+/-40.4 mmol/L。本研究表明，上午肝脏糖原含量显著降低，而小腿肌肉糖原含量的日变化最小。隔夜禁食是一种典型的日常情况，在这种情况下，肝糖原含量较低，而肌肉糖原含量相对较小。
Glycogen in tissues functions not only as carbohydrate reserves, but also as molecular sensors capable of activating signaling pathways in response to physical activity. While glycogen in the skeletal muscles is mainly a local energy substrate, glycogen in the liver serves as a glucose reserve to maintain normal blood glucose levels in the body, even during the sleep state. The aim of this study is to compare the diurnal variation of glycogen in the muscle and liver of human subjects under normal conditions. The glycogen content was measured in the muscle and liver of 10 young, healthy, male volunteers using C-13 MRS, a non-invasive technique. The subjects remained sedentary, and glycogen concentration was measured six times daily. Experimental meals were provided to achieve individual energy balance, estimated according to the energy requirement guideline for patients from Japan. The largest variation in muscle glycogen compared with 1 h after supper (20:00 on Day 1) was 3.1 +/- 8.2 mmol/L (16:00 on Day 2). In the liver, however, the glycogen content decreased by 80.6 +/- 40.4 mmol/L through the overnight fasting period (07:00 on Day 2). This study demonstrated that the glycogen content in the liver was significantly lower in the morning, while the glycogen content in the calf muscles underwent minimal diurnal variation. The overnight fast is a characteristic daily condition, in which liver glycogen content is low, whereas muscle glycogen content is relatively unaffected.