Trigeminal ganglion cells cocultured with gut express vasoactive intestinal peptide.
Trigeminal ganglion cells cocultured with gut express vasoactive intestinal peptide.
复制标题
与肠道共培养的三叉神经节细胞表达血管活性肠肽。
DOI：
10.1016/0012-1606(87)90504-5
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发表时间：
1987
影响因子：
2.7
通讯作者：
Epstein,ML
中科院分区：
文献类型：
--
作者：
Davis,JP;Epstein,ML
神经脊细胞对肾上腺素能和胆碱能递质表型表达的可塑性已经得到了很好的研究。本研究的目的是确定感觉神经节细胞是否具有神经肽递质的可塑性。我们研究了体内不表达神经肽血管活性肠肽(VIP)的三叉神经节细胞，当与含有大量VIP神经元的组织-肠道生长时，三叉神经节细胞是否会表达这种肽。将雏鸡胚胎三叉神经节移植于雏鸡绒毛尿囊膜上7~8天。将外植体固定、切片，进行血管活性肠肽(VIP)免疫反应(IR)、神经丝蛋白免疫反应和核仁标记染色。在组织块的切片上，我们观察到两类神经元：小的(10-13μm)血管活性肠肽免疫反应阳性细胞和大的(25-32μm)的类似于天然三叉神经细胞的血管活性肠肽免疫反应阳性细胞。胚胎心脏移植的三叉神经节或单独移植的三叉神经节缺乏小的VIP-IR细胞，但含有较大的VIP阴性神经元。这些结果表明，与肠道一起生长的三叉神经节细胞可以表达一种在没有肠道或活体情况下不表达的神经肽。因此，胚胎三叉神经节含有神经肽表达的可塑性细胞。
The plasticity of neural crest cells for the expression of adrenergic and cholinergic transmitter phenotypes has been well studied. The object of this study was to determine if cells of a sensory ganglion are capable of neuropeptide transmitter plasticity. We studied whether cells of the trigeminal ganglion, which do not express the neuropeptide vasoactive intestinal peptide (VIP)in vivo, would express this peptide when grown with a tissue, the gut, that contains large numbers of VIP neurons. Embryonic aneural chick rectum was explanted with the embryonic quail trigeminal ganglion on the chorioallantoic membrane of chick hosts for 7–8 days. The explants were fixed, sectioned, and stained for VIP immunoreactivity (IR), for neurofilament protein immunoreactivity, and for the quail nucleolar marker. In sections of the explants we observed two populations of quail neurons: small (10–13 μm) VIP-IR cells and large (25–32 μm) cells lacking VIP-IR and resembling native trigeminal neurons. Trigeminal ganglia explanted with embryonic heart or trigeminal ganglia explanted alone lacked small VIP-IR cells but contained large VIP-negative neurons. These results show that cells of the trigeminal ganglion grown with the gut can express a neuropeptide they do not express in the absence of the gut orin vivo. Thus the embryonic trigeminal ganglion contains cells that are plastic with respect to neuropeptide expression.