The ptl1 gene expressed in the transmitting tissue of Antirrhinum encodes an extensin‐like protein
The ptl1 gene expressed in the transmitting tissue of Antirrhinum encodes an extensin‐like protein
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金鱼草传播组织中表达的 ptl1 基因编码一种类延伸蛋白
DOI：
10.1046/j.1365-313x.1992.t01-14-00999.x
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发表时间：
1992
期刊：
影响因子：
7.2
通讯作者：
H. Dickinson
中科院分区：
文献类型：
--
作者：
T. C. Baldwin;E. Coen;H. Dickinson
ptl1是一种在天鼻花雌蕊传递组织中特异表达的基因，其编码的蛋白与植物延伸蛋白相似。该蛋白富含脯氨酸（28%）和丝氨酸（9%），并含有其他延伸蛋白样蛋白中发现的几种富含脯氨酸的重复氨基酸基序。三个一致的n -糖基化位点的存在表明它可能是糖基化的。RNA印迹显示，ptl1转录物在成熟雌蕊组织中丰富，但在未成熟花蕾和所有其他植物器官中缺失。mRNA的原位定位表明ptl1的表达仅限于花柱和柱头的传递组织。在该蛋白的n端存在一个假定的信号肽，结合其表达模式，表明ptl1产物可能被分泌到传递组织的细胞外基质中。根据目前关于延伸结构和功能的观点，考虑了ptl1产物对透射组织物理性质的可能贡献。
ptl1, a gene expressed specifically in pistil transmitting tissue of Antirrhinum flowers, encodes a protein with similarity to plant extensins. The protein is rich in proline (28%) and serine (9%) and contains several proline-rich repetitive amino acid motifs found in other extensin-like proteins. The presence of three consensus N-glycosylation sites indicates that it is probably glycosylated. RNA blots show that the ptl1 transcript is abundant in mature pistillar tissue but absent from immature flower buds and all other plant organs tested. In-situ localization of mRNA demonstrates that ptl1 expression is confined to the transmitting tissue of the style and stigma. The presence of a putative signal peptide at the N-terminus of the protein, taken together with the expression pattern, indicates that the ptl1 product may be secreted into the extracellular matrix of the transmitting tissue. The possible contributions of the ptl1 product to the physical properties of the transmitting tissue are considered in the light of current views on extensin structure and function.