Wet lipid extraction from the microalga Nannochloropsis sp.: Disruption, physiological effects and solvent screening
Wet lipid extraction from the microalga Nannochloropsis sp.: Disruption, physiological effects and solvent screening
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DOI：
10.1016/j.algal.2016.11.005
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发表时间：
2017-01-01
影响因子：
5.1
通讯作者：
Marchal, Luc
中科院分区：
文献类型：
--
作者：
Angles, Emilie;Jaouen, Pascal;Marchal, Luc
对于生物柴油应用，微藻，特别是微拟球藻被认为是用于脂质生产的有前途的原料。主要问题是高脂质生产率，但也是稳健和节能的下游工艺。在生物精炼过程中，细胞机械预处理和溶剂萃取如今被研究以允许湿路线脂质回收。本研究提出了一种在湿态条件下根据萃取性能筛选油脂萃取溶剂的方法。它包括对部分破碎的微藻细胞悬浮液进行短液/液提取。在一个测试中，其允许鉴定每种溶剂的：（i）溶解脂质化合物的能力;（ii）细胞壁/膜的限制和（iii）动力学。如果使用低水溶性溶剂，则细胞破碎似乎是主要控制步骤。从破碎的微藻的悬浮液中提取脂质比从干燥的生物质中提取更有效（相同的溶剂，相同的能量和时间），并且水的存在增强了饱和脂肪酸回收的选择性。在10 min内破碎率为84%，提取率为50%，饱和脂肪酸（SFA）含量富集到72%。11种溶剂筛选显示，溶剂选择对10 min批次浸提产率有显著影响。根据本研究所考虑的标准，最佳溶剂为甲基叔丁基醚（MTBE）和环戊基甲基醚（CPME）。它们是具有接近目标脂肪酸的汉森溶解度参数、在水中的低溶解度和低蒸发热的那些。它们是氯化溶剂或烷烃的替代品。(C)2016爱思唯尔B. V.保留所有权利。
For biodiesel applications, microalgae and especially Nannochloropsis sp. are considered as a promising feedstock for lipid production. Main issues are high lipid productivity, but also robust and energy efficient downstreamprocesses. In the biorefinery process, cellular mechanical pretreatment and solvent extraction are investigated nowadays to allow wet route lipid recovery. In this study, a method to screen solvents according their performance to extract lipids in wet condition was proposed. It consisted in short liquid/liquid extraction on cells suspension of microalgae partially disrupted. In one test, it allowed identifying for each solvent: (i) the ability to solubilize lipids compounds; (ii) the limitation by the cell wall/membrane and (iii) kinetics. The cell disruption appeared to be the main controlling step if low water soluble solvents were used. Extraction of lipids from suspension of disrupted microalgae was more efficient than extraction from dried biomass (same solvent, same energy and time) and the water presence enhanced the selectivity for saturated fatty acids recovery. 50% extraction yield was achieved with 84% disruption rate in 10 min and saturated fatty acids (SFA) content was enriched to 72% of the extracted lipids. The 11 solvents screening showed a significant impact of the solvent choice on a 10 min batch extraction yield. Based on the criteria considered in this study, the best solvents were Methyl tert-butyl ether (MTBE) and cyclopentylmethyl ether (CPME). They were those with Hansen solubility parameters close to the target fatty acids, low solubility in water and low heat of vaporization. They represent alternatives to chlorinated solvents or alkanes. (C) 2016 Elsevier B.V. All rights reserved.