Antimicrobial activity of tigecycline alone or in combination with rifampin against Staphylococcus epidermidis in biofilm
Antimicrobial activity of tigecycline alone or in combination with rifampin against Staphylococcus epidermidis in biofilm
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DOI：
10.1007/s12223-013-0296-9
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发表时间：
2014-07-01
影响因子：
2.6
通讯作者：
Kaznowski, Adam
中科院分区：
文献类型：
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作者：
Szczuka, Ewa;Kaznowski, Adam
表皮葡萄球菌是健康人体皮肤的寄生菌，但近年来，它已被认为是一种医院病原体，特别是在免疫功能低下的患者。对S.表皮被认为是基于其在医疗设备表面形成生物膜的能力，细菌细胞可能会在医疗设备表面存在，免受宿主防御和抗菌剂的影响。利福平已被证明是根除葡萄球菌生物膜中最有效的抗菌药物之一。然而，这种抗生素不应用于单一疗法。因此，本研究的目的之一是研究替加环素/利福平联合治疗耐甲氧西林沙门氏菌的疗效。包埋在生物膜中的表皮。在80例有临床意义的S.表皮葡萄球菌分离株中，75株具有形成生物膜的能力。这些细菌通过ica依赖性机制形成生物膜。然而，其他生物膜相关基因，包括aap（编码积累相关蛋白）和bhp（编码细胞壁相关蛋白），分别在85%和29%的分离株中存在。对S.还在共聚焦激光扫描显微镜（CLSM）中分析了表皮葡萄球菌菌株，并且获得的图像证明了它们的结构的差异。体外研究表明替加环素对S.在生物膜中生长的表皮细胞的浓度范围为0.125 - 2 μ g/mL。替加环素与利福平联合治疗对包埋在生物膜中的细菌的活性高于替加环素单药治疗。
Staphylococcus epidermidis is a commensal inhabitant of the healthy human skin, but in the recent years, it has been recognized as a nosocomial pathogen especially in immunocompromised patients. The pathogenesis of S. epidermidis is thought to be based on its capacity to form biofilms on the surface of medical devices, where bacterial cells may persist, protected from host defence and antimicrobial agents. Rifampin has been shown to be one of the most active antimicrobial agents in the eradication of the staphylococcal biofilm. However, this antibiotic should not be used in monotherapy. Therefore, one of the objectives of our research was to study the efficacy of the tigecycline/rifampin combination against methicillin-resistant S. epidermidis embedded in biofilms. Of the 80 clinically significant S. epidermidis isolates, 75 strains possess the ability to form a biofilm. These bacteria formed the biofilm via ica-dependent mechanisms. However, other biofilm-associated genes, including aap (encoding accumulation-associated protein) and bhp (coding cell wall-associated protein), were present in 85 and 29 % of isolates, respectively. The biofilm structures of S. epidermidis strains were also analyzed in confocal laser scanning microscopy (CLSM) and the obtained image demonstrated differences in their architecture. In vitro studies showed that the MIC value for tigecycline against S. epidermidis growing in the biofilm ranged from 0.125 to 2 mu g/mL. Tigecycline in combination with rifampin demonstrated higher activity against bacteria embedded in biofilms than tigecycline alone.