Interaction of a 14-3-3 protein with the plant microtubule-associated protein EDE1.
Interaction of a 14-3-3 protein with the plant microtubule-associated protein EDE1.
复制标题
14-3-3 蛋白与植物微管相关蛋白 EDE1 的相互作用。
DOI：
10.1093/aob/mcr050
复制
发表时间：
2011
期刊：
影响因子：
4.2
通讯作者：
Pignocchi C
中科院分区：
文献类型：
--
作者：
Pignocchi C
Background and AimsThe cell cycle-regulated protein ENDOSPERM DEFECTIVE 1 (EDE1) is a novel plant microtubule-associated protein essential for plant cell division and for microtubule organization in endosperm. EDE1 is only present on microtubules at mitosis and its expression is highly cell cycle regulated both at the protein and the transcript levels.MethodsTo search for EDE1-interacting proteins, a yeast two-hybrid screen was used in which EDE1 was fused with GAL4 DNA binding domain and expressed in a yeast strain that was then mated with a strain carrying a cDNA library fused to the GAL4 transactivation domain. Candidate interacting proteins were identified and confirmedin vitro.Key Results14-3-3 upsilon was isolated several times from the library screen. Inin vitrotests, it also interacted with EDE1: 14-3-3 upsilon most strongly associates with EDE1 in its free form, but also weakly when EDE1 is bound to microtubules. This study shows that EDE1 is a cyclin-dependent kinase substrate but that phosphorylation is not required for interaction with 14-3-3 upsilon.ConclusionsThe results suggest that 14-3-3 proteins may play a role in cytoskeletal organization of plant cells. The potential role of this interaction in the dynamics of EDE1 during the cell cycle is discussed.
