Positron emission tomography: A real-time tool to quantify early islet engraftment in a preclinical large animal model
Positron emission tomography: A real-time tool to quantify early islet engraftment in a preclinical large animal model
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DOI：
10.1097/01.tp.0000284730.86567.9f
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发表时间：
2007-10-15
期刊：
影响因子：
6.2
通讯作者：
Lundgren, Torbjorn
中科院分区：
文献类型：
--
作者：
Eich, Torsten;Eriksson, Olof;Lundgren, Torbjorn
背景资料。临床胰岛移植目前正在探索作为治疗I型糖尿病和低血糖意识不清的人的一种选择。迫切需要监测移植物存活的技术来优化这一过程。因此，本研究的目的是开发一种对移植胰岛在移植周围和移植后早期阶段的存活进行成像的技术。将分离的猪胰岛体外标记2-脱氧-2[F-18]氟-D-葡萄糖([F-18]FDG)，注入麻醉猪(n=10)。在正电子发射断层扫描/计算机断层扫描混合系统上进行动态检查。在移植时，超过95%的放射性局限于胰岛。在胰岛输注结束时，肝脏内输注放射性的峰值百分比仅为54+/-5.1%。放射性在肝脏的分布是不均匀的。全身检查未见肺或脑内蓄积，肝外放射性除尿液外均在猪体内分布。我们的结果表明，门脉内移植后几分钟内，几乎50%的胰岛受损，所含的FDG被释放。未在脑内蓄积的放射性分布表明，活性是以[F-18]FDG-6P的形式从裂解的胰岛细胞释放出来的，而不是以天然的[F-18]FDG的形式释放。这项技术有望成为一种强大的定量工具，在临床上随时可用来评估最初的胰岛植入和存活。
Background. Clinical islet transplantation is currently being explored as a therapeutic option for persons with type I diabetes and hypoglycemic unawareness. Techniques to monitor graft survival are urgently needed to optimize the procedure. Therefore, the objective of the present study was to develop a technique for imaging survival of transplanted islets in the peritransplant and early posttransplant phase.Methods. Isolated porcine islets were labeled in vitro with 2-deoxy-2 [F-18]fluoro-D-glucose ([F-18]FDG) and infused intraportally into anesthetized pigs (n=10). Dynamic examination was performed on a positron emission tomography/ computed tomography hybrid system.Results. More than 95% of the radioactivity was confined to the islets at the time of transplantation. The peak percentage of infused radioactivity within the liver, quantified at the end of the islet infusion, was only 54 +/- 5.1%. The distribution of the radioactivity in the liver was found to be heterogeneous. A whole-body examination showed no accumulation in the lungs or brain; extrahepatic radioactivity was, except urinary excretion, evenly distributed in the pig body.Conclusions. Our results imply that almost 50% of the islets were damaged to the extent that the FDG contained was release within minutes after intraportal transplantation. The distribution of radioactivity without accumulation in the brain indicates that the activity is released from lysed islet cells in the form of [F-18] FDG-6P rather than native [F-18] FDG. The presented technique shows promise to become a powerful and quantitative tool, readily available in the clinic, to evaluate initial islet engraftment and survival.