Membrane-cytoskeleton associations during myogenesis deviate from traditional definitions.
Membrane-cytoskeleton associations during myogenesis deviate from traditional definitions.
复制标题
肌发生过程中的膜-细胞骨架关联偏离了传统定义。
DOI：
10.1016/0014-4827(89)90414-x
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发表时间：
1989
影响因子：
3.7
通讯作者：
Kaufman,SJ
中科院分区：
文献类型：
--
作者：
Lowrey,AA;Kaufman,SJ
质膜-细胞骨架协会涉及四个膜蛋白（A5，H58，H36和120）在开发L8E63大鼠骨骼肌细胞，使用免疫荧光显微镜和光度法的基础上研究了三个标准：曲拉通不溶性，共定位与细胞骨架成分，和敏感性的cytokeron-directed药物。结果表明，在骨骼肌发生过程中，膜蛋白和细胞骨架之间存在发育阶段特异性的关联。发现了几个不一致的传统预期的膜细胞骨架协会。例如，尽管A5是Triton不溶性的并且对细胞松弛素敏感，但其分布通常不符合任何已知的细胞骨架结构。此外，A5的形貌取决于质膜的完整性。H36和120完全溶于Triton，因此根据公认的定义，预期不会与任何细胞骨架组分相关。然而，H36和肌动蛋白codrupture在细胞松弛素的存在下，而I20，其分布不对应于微管，是唯一敏感的破坏。这些结果表明：（i）单独的Triton溶解度和共定位都不能预测所有的膜-细胞骨架缔合，膜和细胞骨架之间的某些缔合在非离子去污剂中是不稳定的;（ii）蛋白质在膜中的天然分布可能不能反映它们的细胞骨架缔合;和（iii）一些与细胞骨架没有明显关联的膜蛋白的形貌可能受到细胞骨架的极大影响。
Plasma membrane-cytoskeleton associations involving four membrane proteins (A5, H58, H36, and 120) were studied in developing L8E63 rat skeletal muscle cells using immunofluorescence microscopy and photometry on the basis of three criteria: Tritoninsolubility, colocalization with cytoskeletal components, and sensitivity to cytoskeleton-directed drugs. The results presented demonstrate that there are developmental stagespecific associations between membrane proteins and the cytoskeleton during skeletal myogenesis. Several inconsistencies were found with traditional expectations of membrane-cytoskeleton associations. For example, although A5 is Triton-insoluble and sensitive to cytochalasin, its distribution generally does not correspond with any known cytoskeletal structure. Furthermore, the topography of A5 is dependent on the integrity of the plasma membrane. H36 and 120 are completely soluble in Triton and therefore by accepted definitions would not be expected to be associated with any cytoskeletal component. Yet H36 and actin codisrupt in the presence of cytochalasin, while I20, whose distribution does not correspond with microtubules, is uniquely sensitive to their disruption. These results demonstrate that (i) neither Triton-solubility nor colocalization alone predicts all membrane-cytoskeleton associations; some associations between the membrane and cytoskeleton are unstable in nonionic detergent; (ii) the native distribution of proteins in the membrane may not reflect their cytoskeletal associations; and (iii) the topography of some membrane proteins with no apparent association with the cytoskeleton may be greatly influenced by the cell cytoskeleton.