RESOLUTION IN COUNTERCURRENT CHROMATOGRAPHY
RESOLUTION IN COUNTERCURRENT CHROMATOGRAPHY
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DOI：
10.1080/01483918508074125
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发表时间：
1985-01-01
期刊：
影响因子：
--
通讯作者：
ITO, Y
中科院分区：
文献类型：
--
作者：
CONWAY, WD;ITO, Y
作为一个例子，在非行星逆流色谱分离的DNP-谷氨酸和DNP-丙氨酸，它表明，分辨率，Rs，溶质对可以令人满意地预测从知识的柱效率，N，固定相，SF，和分配系数的物质在溶剂系统中所占的柱体积的分数。分离度是相体积比的函数，SF的增加有利于快速洗脱化合物的分离。分配系数K、分离因子α和柱效N与SF无关。增加SF会通过增加容量因子k1来增加RS。对于SF为0.4的水平流通CCC的特征，当N = 1000时，第一洗脱物质K1的值约为0.5，或当N = 100时，K1的值约为10，其中α为2时，获得基线分离（RS= 1.5）。将SF增加到0.8，多层线圈的特征，高速CCC，有利于快速洗脱溶质的分辨率，那些具有低K值的溶质。然后获得基线分辨率，对于N = 1000，K1约为0.07，或者对于N = 100，K1约为1.5。
Using as an example the separation of DNP-glutamic acid and DNP-alanine in a nonplanetary countercurrent chromatograph, it was demonstrated that resolution, Rs, of a solute pair can be satisfactorily predicted from knowledge of the column efficiency, N, the fraction of column volume occupied by stationary phase, SF, and the partition coefficients of the substances in the solvent system employed. Resolution is a function of the phase volume ratio and separation of rapidly eluted compounds is favored by an increase in SF. The partition coefficients, K, the separation factor, α, and the column efficiency, N, are independent of SF. Increasing SFbrings about an increase in RSby increasing the capacity factor k1. For SFof 0.4, characteristic of the horizontal flow-through CCC, baseline separation (RS= 1.5) is obtained for values of K1, the first eluted substance, of about 0.5 for N = 1000 or K1of 10 for N = 100, where α is 2. Increasing SFto 0.8, characteristic of the multilayer coil, high speed CCC, favors resolution of rapidly eluted solutes, those with low K values. Baseline resolution is then obtained for K1of about 0.07 with N = 1000 or for K1of about 1.5 for N = 100.