Movement and fixation of intestinal microbiota after administration of human feces to germfree mice
Movement and fixation of intestinal microbiota after administration of human feces to germfree mice
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DOI：
10.1128/aem.71.6.3171-3178.2005
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发表时间：
2005-06-01
影响因子：
4.4
通讯作者：
Benno, Y
中科院分区：
文献类型：
--
作者：
Kibe, R;Sakamoto, M;Benno, Y
人类菌群相关（HFA）小鼠被认为是研究人类肠道细菌生态和代谢的工具，尽管它们作为模型有一些局限性。通过16 S rRNA基因序列和末端限制性片段长度多态性（T-RFLP）分析，揭示了人类肠道菌群给药后HFA小鼠肠道菌群优势种的变化。将特征性末端限制性片段（T-RF）定量为所有T-RF总峰面积的比例。在这项研究中，只有在bp 366处的T-RF峰的比例减少，该峰被鉴定为γ-变形菌纲和Coriobacteriaceae科。在bp 56、184和196处随时间增加的T-RF与梭菌组有关。然而，大多数分离的细菌具有独特的群体转移是细菌型。小鼠后代肠道菌群的垂直传播也通过来自样品之间的T-RFLP模式的相似性的树状图分析进行了研究。因此，HFA小鼠及其后代的肠道微生物群反映了个体人类肠道细菌的组成，并进行了一些修改。此外，我们发现，人源性乳酸杆菌（HDL），这已被认为是难以在HFA小鼠肠道在以前的研究中的基础上的培养方法，可以检测到HFA小鼠肠道中使用乳酸菌特异性引物和HDL特异性引物。我们的研究结果表明，HFA小鼠的肠道微生物群代表了来自人类来源的有限细菌样本，并通过宿主和细菌之间的未知相互作用进行选择。
Human flora-associated (HFA) mice have been considered a tool for studying the ecology and metabolism of intestinal bacteria in humans, although they have some limitations as a model. Shifts in dominant species of microbiota in HFA mice after the administration of human intestinal microbiota was revealed by 16S rRNA gene sequence and terminal restriction fragment length polymorphism (T-RFLP) analyses. Characteristic terminal restriction fragments (T-RFs) were quantified as the proportion of total peak area of all T-RFs. Only the proportion of the T-RF peak at bp 366, identified as the Gammmaproteobacteria group and the family Coriobacteriaceae, was reduced in this study. Increased T-RFs over time at bp 56, 184, and 196 were affiliated with the Clostridium group. However, most of the isolated bacteria with unique population shifts were phylotypes. The vertical transmission of the intestinal microbiota of the mouse offspring was also investigated by dendrogram analysis derived from the similarity of T-RFLP patterns among samples. As a result, the intestinal microbiota of HFA mice and their offspring reflected the composition of individual human intestinal bacteria with some modifications. Moreover, we revealed that human-derived lactobacilli (HDL), which have been considered difficult to colonize in the HFA mouse intestine in previous studies based on culture methods, could be detected in the HFA mouse intestine by using a lactic acid bacterium-specific primer and HDL-specific primers. Our results indicate that the intestinal microbiota of HFA mice represents a limited sample of bacteria from the human source and are selected by unknown interactions between the host and bacteria.