Comparative growth dynamics and morphology between cultured myofibroblasts from granulating wounds and dermal fibroblasts.
Comparative growth dynamics and morphology between cultured myofibroblasts from granulating wounds and dermal fibroblasts.
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肉芽伤口培养的肌成纤维细胞和真皮成纤维细胞的生长动力学和形态的比较。
DOI：
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发表时间：
1984
影响因子：
6
通讯作者：
M. Woodward
中科院分区：
文献类型：
--
作者：
J. V. Berg;R. Rudolph;M. Woodward
Rat myofibroblasts from granulating wound biopsies (RGW) were successfully cultured and compared in terms of growth and morphology with fibroblasts from uninjured rat dermis (RD). Populations of early passage (P-3) RGW myofibroblasts grew significantly more slowly than RD fibroblasts. Logarithmic growth was nearly the same in late passage (P-30) populations of both cell types. Early passage RGW myofibroblasts were similar to those in vivo, as shown by well-defined microfilament bundles. RD fibroblasts contained less well defined microfilaments. Both late passage RGW myofibroblasts and RD fibroblasts displayed evidence of morphologic dedifferentiation. These data show that morphologic features of myofibroblasts and fibroblasts in vivo are maintained in vitro. Evidence is presented that cultured animal myofibroblasts maintain differentiation in early passage, whereas late passage cells suggest that these differences disappear with time.
