Determination of cichoric acid content in dried press juice of purple coneflower (Echinacea purpurea) with capillary electrophoresis
Determination of cichoric acid content in dried press juice of purple coneflower (Echinacea purpurea) with capillary electrophoresis
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DOI：
10.1016/j.talanta.2005.01.028
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发表时间：
2005-06-15
期刊：
影响因子：
6.1
通讯作者：
Kreft, S
中科院分区：
文献类型：
--
作者：
Mancek, B;Kreft, S
紫锥菊（Echinacea purpurea）是一种免疫刺激药物，含有多种物质。活性中最重要的是多糖、咖啡酸衍生物（菊苣酸）、烷酰胺和糖蛋白。目前还不清楚哪些物质负责活动。菊苣酸是评价E.本文建立了一种改进的毛细管电泳法测定紫锥花干榨汁中菊苣酸的方法。最佳条件为：电泳缓冲液-75 mM硼酸盐，pH 8.8;进样20 mbar持续20 s;在20 kV下分离;在350 nm下检测，温度35 ℃。(c)2005 Elsevier B. V.保留所有权利。
Purple coneflower (Echinacea purpurea) is an immunostimulating drug, containing multiple substances. The most important in activity are polysaccharides, caffeic acid derivatives (cichoric acid), alkamides and glycoproteins. It is not clear yet, which substances are responsible for activity. Cichoric, acid is an appropriate marker of the quality of E. purpurea containing product, because it has immune stimulatory effects and it is susceptible to degradation.In this work, an improved capillary electrophoresis method for determining cichoric acid in dried press juice from purple coneflower was developed. The optimal conditions were: electrophoretic buffer-75 mM borate, pH 8.8; injection 20 mbar for 20 s; separation at 20 kV; detection at 350 nm, temperature 35 degrees C. (c) 2005 Elsevier B.V. All rights reserved.