In Vivo Visualization and Function Probing of Transport mRNPs Using Injected FIT Probes.
In Vivo Visualization and Function Probing of Transport mRNPs Using Injected FIT Probes.
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DOI：
10.1007/978-1-4939-7213-5_18
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发表时间：
2018
影响因子：
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通讯作者：
J. Chamiolo;Imre Gáspár;A. Ephrussi;O. Seitz
中科院分区：
文献类型：
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作者：
J. Chamiolo;Imre Gáspár;A. Ephrussi;O. Seitz
荧光杂交方法，如使用FIT探针，能够在体内检测从其内源性、遗传非修饰基因座转录的特异性mRNA。在这里，我们描述了抗核酸酶的FIT探针的设计、合成和注射到发育中的果蝇细胞中，以检测内源性定位mRNA作为威尔斯孔，以探测结构RNA元件的功能。
Fluorogenic hybridization methods, such as the use of FIT probes, enable the in vivo detection of specific mRNAs transcribed from their endogenous, genetically nonmodified loci. Here, we describe the design, synthesis and injection of nuclease resistant FIT probes into developingDrosophilaoocytes to detect endogenous localizing mRNAs as wells as to probe function of structural RNA elements.