Selective Control of the Prorocentrum minimum Harmful Algal Blooms by a Novel Algal-Lytic Bacterium Pseudoalteromonas haloplanktis AFMB-008041
Selective Control of the Prorocentrum minimum Harmful Algal Blooms by a Novel Algal-Lytic Bacterium Pseudoalteromonas haloplanktis AFMB-008041
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DOI：
10.1007/s10126-008-9167-9
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发表时间：
2009-07-01
影响因子：
3
通讯作者：
Lee, Choul-Gyun
中科院分区：
文献类型：
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作者：
Kim, Jeong-Dong;Kim, Ji-Young;Lee, Choul-Gyun
在这项研究中，我们研究了从韩国马山湾表层海水中分离出来的盐浮游假交替单胞菌 AFMB-08041 的溶藻活性和生物控制机制。此外，我们评估了 AFMB-08041 是否可以用作生物防治剂来调节有害甲藻原甲藻的最小量。从这些实验中，我们发现以2.5 x 10(4) cfu ml(-1)的最终密度接种AFMB-08041会导致P.minimum细胞在5天内降解（> 90%）。通过 AFMB-08041 菌株的间接攻击，藻类细胞被裂解。我们的结果还表明 AFMB-08041 产生的溶藻化合物可能具有 β-葡萄糖苷酶活性。然而，P. haloplanktis AFMB-08041 无法抑制其他藻类的生长，例如塔玛亚历山大藻、Akashiwo sanguinea、Cochlodinium polykrikoides、Gymnodinium catenatum 和 Heterosigma akashiwo。此外，我们观察到与P. minimum形态结构非常相似的Prorocentrum dentatatum的生长也被P. haloplanktis AFMB-08041有效抑制。因此，AFMB-08041 对 P.minimum 降解的影响似乎是物种特异性的。在室内中生态系统中进行测试时，P. haloplanktis AFMB-08041 在接种后 5 天内将活的最小 P. 细胞数量减少了 94.5%。这项研究的综合结果清楚地表明，这种细菌能够调节 P. minimum 的有害藻华。此外，这些结果将使我们能够制定一种新的策略来人为控制自然界中有害的藻华形成物种。
In this study, we examined the algal-lytic activities and biological control mechanisms of Pseudoalteromonas haloplanktis AFMB-08041, which was isolated from surface seawater obtained at Masan Bay in Korea. In addition, we assessed whether AFMB-08041 could be used as a biocontrol agent to regulate harmful dinoflagellate Prorocentrum minimum. From these experiments, we found that the inoculation of AFMB-08041 at a final density of 2.5 x 10(4) cfu ml(-1) caused P. minimum cells to degrade (> 90%) within 5 days. The algal cells were lysed through an indirect attack by the AFMB-08041 bacterial strain. Our results also suggest that the algal-lytic compounds produced by AFMB-08041 may have beta-glucosidase activity. However, P. haloplanktis AFMB-08041 was not able to suppress the growth of other alga such as Alexandrium tamarense, Akashiwo sanguinea, Cochlodinium polykrikoides, Gymnodinium catenatum, and Heterosigma akashiwo. Moreover, we observed that the growth of Prorocentrum dentatum, which has a very similar morphological structure to P. minimum, was also effectively suppressed by P. haloplanktis AFMB-08041. Therefore, the effect of AFMB-08041 on P. minimum degradation appears to be species specific. When testing in an indoor mesocosms, P. haloplanktis AFMB-08041 reduced the amount of viable P. minimum cells by 94.5% within 5 days after inoculation. The combined results of this study clearly demonstrate that this bacterium is capable of regulating the harmful algal blooms of P. minimum. In addition, these results will enable us to develop a new strategy for the anthropogenic control of harmful algal bloom-forming species in nature.