Enhancing the stability of astaxanthin by encapsulation in poly (l-lactic acid) microspheres using a supercritical anti-solvent process
Enhancing the stability of astaxanthin by encapsulation in poly (l-lactic acid) microspheres using a supercritical anti-solvent process
复制标题
使用超临界反溶剂工艺封装在聚（L-乳酸）微球中提高虾青素的稳定性
DOI：
10.1016/j.partic.2018.04.006
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发表时间：
2019-06-01
期刊：
影响因子：
3.5
通讯作者：
Jiang, Yanbin
中科院分区：
文献类型：
--
作者：
Liu, Guijin;Hu, Man;Jiang, Yanbin
为改善虾青素的理化性质，以二氯甲烷/丙酮混合物为溶剂，超临界二氧化碳为抗溶剂，采用超临界抗溶剂（SAS）工艺将其包封在聚（L - 乳酸）（PLLA）中。采用正交试验设计研究了改变溶剂比例、温度、压力、载体浓度和流速这五种SAS操作条件对颗粒微观结构的影响。在最佳条件下，制备出虾青素/PLLA颗粒，其包封率为91.5%，平均粒径为954.6 nm。扫描电子显微镜（SEM）图像显示，大多数虾青素/PLLA颗粒为均匀的微球。傅里叶变换红外光谱（FT - IR）表明，SAS工艺未改变虾青素的化学结构。色差、X射线衍射、热重和差示扫描量热分析结果表明，虾青素以无定形状态包封在PLLA基质中。总体而言，虾青素/PLLA微球极大地提高了虾青素在储存过程中的稳定性，且残留溶剂水平远低于国际协调会议（ICH）规定的限值。这意味着采用SAS制备的虾青素/PLLA微球在许多食品、化妆品和药物配方中具有巨大的应用潜力。（C）2018中国颗粒学会和中国科学院过程工程研究所。由爱思唯尔出版集团出版。保留所有权利。
To improve the physicochemical properties of astaxanthin, it was encapsulated in poly (I-lactic acid) (PLLA) using a supercritical anti-solvent (SAS) process with dichloromethane/acetone mixture as the solvent, and supercritical CO2 as the anti-solvent. The effects of altering five SAS operating conditions, solvent ratio, temperature, pressure, concentration of carrier, and flow rate, on the microstructure of particles were investigated using an orthogonal experimental design. Under the optimal conditions, astaxanthin/PLLA particles were produced with an encapsulation efficiency of 91.5% and a mean particle size of 954.6 nm. SEM images showed that most astaxanthin/PLLA particles were uniform microspheres. FT-IR spectra showed that the chemical structure of astaxanthin was unchanged by the SAS process. The results of chromatic difference, X-ray diffraction, thermogravimetric, and differential scanning calorimetry analyses showed that astaxanthin had been encapsulated in the PLLA matrix in an amorphous state. Overall, astaxanthin/PLLA microspheres greatly enhanced the stability of astaxanthin during storage, and the levels of residual solvents were far lower than the ICH limits. This means that astaxanthin/PLLA micro spheres prepared using SAS show great potential for use in many food, cosmetic, and pharmaceutical formulations. (C) 2018 Chinese Society of Particuology and Institute of Process Engineering, Chinese Academy of Sciences. Published by Elsevier B.V. All rights reserved.