Electrical properties of extracted rat liver tissue
Electrical properties of extracted rat liver tissue
复制标题
提取的大鼠肝组织的电特性
DOI：
10.1007/bf02576787
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Y. Monden
中科院分区：
文献类型：
--
作者：
Y. Konishi;T. Morimoto;Y. Kinouchi;T. Iritani;Y. Monden
我们试图研究的过程中，缺血诱导的干扰，在大鼠肝脏，采用生物电阻抗技术。在不同的孵育温度下，用4电极法连续测量6个雄性Wistar大鼠肝脏样品的生物电阻抗。生物组织的电特性可以用三个参数来表示：细胞外电阻（Re）、细胞内电阻（Ri）和细胞膜电容（Cm）。使用计算机程序，通过曲线拟合技术从电阻抗的测量值计算这三个参数。取肝后Re值迅速升高，然后缓慢下降。在36°C下约13 min后，Revalue达到峰值，然后缓慢下降，3 h后趋于恒定。Re的Tmax（Re达到最大值的时间）与孵育温度呈负相关（R=-0.973，P <0.001）。在36°C下浸提4 h后，Ri值在浸提初期下降一次，随后几乎没有变化，随后又有所上升。Cm的变化规律与Re值相似，Cm的Tmax与孵育温度呈负相关（R=-0.969，P <0.001）。Re和Cm值的增加，以及在血流停止后相当长的时间内Ri值的降低，表明细胞外液的阻力由于其体积的减少而增加，细胞膜电容由于细胞肿胀而增加，以及细胞液阻力由于其体积的增加而减少。当Cm值在峰值之后的初始逐渐降低之后快速降低时的时间与当Ri值开始增加时的时间很好地对应，由此估计细胞裂解进行，并且细胞外液在此时开始流入细胞。这项研究的结果表明，估计由于缺血肝组织或组织结构的变化的可能性。
We attempted to investigate the process of ischemia-induced disturbances in the rat liver, employing the electrical bio-impedance technique. The electrical bio-impedance was measured continuously over 6h by the 4-electrode method, at various incubation temperatures, in six liver samples extracted from male Wistar rats. The electrical properties of biological tissues can be expressed in terms of three parameters: extracellular resistance (Re), intracellular resistance (Ri) and cell membrane capacitance (Cm). These three parameters were calculated from the measured values of the electrical impedance by the curve-fitting technique, using a computer program. The Re value increased rapidly after the rat livers were extracted, and then decreased slowly. The Revalue reached a peak after about 13 min at 36°C, and then decreased slowly, becoming constant after 3h. There was a negative correlation between the Tmax of Re (the time when Re reached a maximum) and the incubation temperature (R=−0.973,P<0.001). The Ri value decreased once in the early stage after extraction, followed by almost no change and then an increase after 4h at 36°C. The Cm showed a similar pattern of change to the Re value, and a negative correlation was also found between the Tmax of Cm and the incubation temperature (R=−0.969,P<0.001). The increases in the Re and Cm values, and the decrease in the Ri value for quite long periods after the blood flow has stopped, suggest an increase in the resistance of extracellular fluid due to a decrease in its volume, an increase in cell membrane capacitance due to cell swelling, and a decrease in cellular fluid resistance due to an increase in its volume. The time when the Cm value decreases rapidly after an initial gradual decrease after the peak corresponds well with the time when the Ri value begins to increase, from which it is estimated that cell lysis proceeds and that the flow of extracellular fluid into the cell begins at this time. The findings of this study suggest the possibility of estimating the changes in liver tissue or the tissue structure due to ischemia.
通过 31P-NMR 光谱测量大鼠肝脏的体内代谢状态。
DOI：
10.1016/0167-4889(86)90032-7
发表时间：
1986
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Malloy,CR;Cunningham,CC;Radda,GK
通讯作者：
Radda,GK