Transcriptomic analysis reveals different responses to ammonia stress and subsequent recovery between Coilia nasus larvae and juveniles
Transcriptomic analysis reveals different responses to ammonia stress and subsequent recovery between Coilia nasus larvae and juveniles
复制标题
转录组分析揭示了鲚幼虫和幼鱼对氨胁迫的不同反应以及随后的恢复
DOI：
10.1016/j.cbpc.2020.108710
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发表时间：
2020-04-01
影响因子：
3.9
通讯作者：
Xu, Pao
中科院分区：
文献类型：
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作者：
Gao, Jun;Zhu, Yongxiang;Xu, Pao
过量的氨会对水生动物的健康、生长和大量死亡造成负面影响，特别是在不同的发育时期。然而，对鱼类幼体和幼鱼对氨胁迫的潜在反应的研究要少得多。对氨胁迫和随后在淡水中恢复的Coilia nasus幼体和幼体进行了转录转录分析。总共获得了958,213,132个干净的读数。共组装了234,830个非基因区，平均长度为1397个碱基，N50为2521个碱基。在鼻甲幼虫和幼虫中分别鉴定出831和952个DEGS。转录分析表明，与嘌呤代谢、免疫、炎症、表观遗传修饰和神经传导相关的基因在鼻甲幼虫和幼虫之间呈现不同的表达趋势。RT-qPCR检测到的其他与嘌呤代谢和表观遗传修饰相关的基因(DNMT1、DNMT3a和Dnmt3b)也表现出不同的表达趋势。这些结果表明，氨的解毒策略和基因调控模式在鼻甲幼体和幼体中是不同的。氨应激可导致炎症反应和免疫功能下降，高表达TNF-α、ILF-2和ILF-3，降低LZM、AKP和ACP活性。此外，氨胁迫严重影响了中华绒螯蟹幼体的神经传导。此外，淡水中的恢复对神经传导也有积极的影响。但值得注意的是，淡水恢复后仍存在免疫力下降和炎症现象。综上所述，我们的研究将有助于揭示幼体和幼体对氨胁迫的不同反应。
Excessive ammonia triggered negative effects on aquatic animals' health, growth, and mass death, especially at different developmental periods. However, the underlying responses to ammonia stress in fish larvae and juveniles were much less explored. Transcriptomic analysis of Coilia nasus larvae and juveniles treated with ammonia stress and subsequent recovery in freshwater were performed. Total 958,213,132 clean reads were obtained. A total of 234,830 unigenes with an average length of 1397 bp and N50 value 2521 bp were assembled. 831 and 952 DEGs were identified in C. nasus larvae and juveniles, respectively. Transcriptomic analysis revealed that genes associated with purine metabolism, immune, inflammation, epigenetic modification, and nerve conduction presented different expression trends between C. nasus larvae and juveniles. Other genes related to purine metabolism (XDH) and epigenetic modifications (DNMT1, DNMT3A, and DNMT3B) detected by RT-qPCR also displayed different expression trends. These results indicated that ammonia detoxify strategies and gene regulation patterns were different in C. nasus larvae and juveniles. Higher TNF-alpha, ILF-2, and ILF-3 expression and reduced LZM, AKP, and ACP activities suggested that inflammation and declined immunity were triggered by ammonia stress. Additionally, nervous conduction was severely affected under ammonia stress in C. nasus juveniles. Furthermore, recovery in freshwater had positive effects on nervous conduction. However, it was worth noting that reduced immunity and inflammation were still existed after recovery in freshwater. In conclusion, our study would be beneficial to reveal the different responses to ammonia stress between larvae and juveniles.