Interactive effects of central leptin and peripheral fuel oxidation on estrous cyclicity.
Interactive effects of central leptin and peripheral fuel oxidation on estrous cyclicity.
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DOI：
10.1152/ajpregu.1999.277.4.r1020
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发表时间：
1999-10
期刊：
影响因子：
--
通讯作者：
J. E. Schneider;Dan Zhou
中科院分区：
文献类型：
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作者：
J. E. Schneider;Dan Zhou
48小时的禁食期抑制叙利亚仓鼠的发情周期，禁食诱导的乏情期可以通过在禁食期间用瘦素进行脑室内治疗来预防。在本实验中，脑室内瘦素的作用被阻断代谢燃料氧化抑制剂的全身治疗。在发情周期的第1天和第2天禁食期间，向侧脑室持续输注瘦素（1 μg/天）。腹腔注射2-脱氧-D-葡萄糖（2DG）用于阻断中枢和外周葡萄糖氧化，并在禁食和瘦素治疗期间用棕榈酸甲酯（MP）灌胃治疗以抑制外周长链脂肪酸氧化。在自由采食的仓鼠中，以不诱导乏情的剂量给予2DG或MP。尽管中枢瘦素水平升高，但2DG或MP处理的仓鼠出现禁食诱导的乏情。因此，当燃料氧化被抑制时，升高的脑室内瘦素浓度不是正常发情周期的充分条件。这些结果提高了中枢瘦素通过间接影响外周燃料代谢来影响生殖的可能性。
A 48-h period of fasting inhibits estrous cycles in Syrian hamsters, and fasting-induced anestrus can be prevented by intracerebroventricular treatment with leptin during the fasting period. In the present experiment, the effects of intracerebroventricular leptin were blocked by systemic treatment with inhibitors of metabolic fuel oxidation. Leptin was infused continuously into the lateral ventricles (1 μg/day) during fasting on days 1 and 2 of the estrous cycle. Intraperitoneal injection of 2-deoxy-d-glucose (2DG) was used to block both central and peripheral glucose oxidation, and intragastric treatment with methyl palmoxirate (MP) was used to inhibit peripheral long-chain fatty acid oxidation during the fasting and leptin-treatment period. 2DG or MP were administered at doses that did not induce anestrus in ad libitum-fed hamsters. Despite elevated central levels of leptin, fasting-induced anestrus occurred in hamsters treated with either 2DG or MP. Thus an elevated intracerebroventricular leptin concentration is not a sufficient condition for normal estrous cycles when fuel oxidation is inhibited. These results raise the possibility that central leptin influences reproduction by indirect effects on peripheral fuel metabolism.