Transcriptome divergence between introduced and native populations of Canada thistle, Cirsium arvense
Transcriptome divergence between introduced and native populations of Canada thistle, Cirsium arvense
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DOI：
10.1111/nph.12258
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发表时间：
2013-07-01
期刊：
影响因子：
9.4
通讯作者：
Rieseberg, Loren H.
中科院分区：
文献类型：
--
作者：
Guggisberg, Alessia;Lai, Zhao;Rieseberg, Loren H.
引进的植物在引进后可能很快进化出新的适应性性状。加拿大蓟（Cirsiumarvense- Cardueae，菊科）是世界上最严重的入侵杂草之一.本研究的目的是比较该物种的本地（欧洲）和引入（北美）种群的基因表达谱，以阐明可能导致这种快速适应的遗传机制。我们使用包含63690个表达序列标签（EST）的定制微阵列芯片探索了10个群体（每个范围5个）的燕麦在3种处理（对照、营养缺乏和遮光）下的转录组，并通过实时定量PCR验证了13个位点的表达水平。只有2116个EST（3.5%）被发现在范围之间差异表达，4458个EST（7.1%）表现出显著的治疗范围效应。其中之一是过度代表的位点参与刺激和压力反应。小蓟在各大洲进化出不同的生活史策略。这两个范围显着不同的R-蛋白介导的防御，对非生物胁迫的敏感性，和发育时机。来自中西部的基因型表现出与其余北美样本不同的表达动力学，这一事实进一步证实了新大陆独立地被殖民了两次的假设。
Introduced plants may quickly evolve new adaptive traits upon their introduction. Canada thistle (Cirsium arvense - Cardueae, Asteraceae) is one of the worst invasive weeds worldwide. The goal of this study is to compare gene expression profiles of native (European) and introduced (North American) populations of this species, to elucidate the genetic mechanisms that may underlie such rapid adaptation. We explored the transcriptome of ten populations (five per range) of C.arvense in response to three treatments (control, nutrient deficiency and shading) using a customized microarray chip containing 63690 expressed sequence tags (ESTs), and verified the expression level of 13 loci through real-time quantitative PCR. Only 2116 ESTs (3.5%) were found to be differentially expressed between the ranges, and 4458 ESTs (7.1%) exhibited a significant treatment-by-range effect. Among them was an overrepresentation of loci involved in stimulus and stress responses. Cirsium arvense has evolved different life history strategies on each continent. The two ranges notably differ with regard to R-protein mediated defence, sensitivity to abiotic stresses, and developmental timing. The fact that genotypes from the Midwest exhibit different expression kinetics than remaining North American samples further corroborates the hypothesis that the New World has been colonized twice, independently.