HIGH-SENSITIVITY TRACER DETECTION USING HIGH-PRECISION GAS-CHROMATOGRAPHY COMBUSTION ISOTOPE RATIO MASS-SPECTROMETRY AND HIGHLY ENRICHED [U-C-13]-LABELED PRECURSORS
HIGH-SENSITIVITY TRACER DETECTION USING HIGH-PRECISION GAS-CHROMATOGRAPHY COMBUSTION ISOTOPE RATIO MASS-SPECTROMETRY AND HIGHLY ENRICHED [U-C-13]-LABELED PRECURSORS
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DOI：
10.1021/ac00034a004
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发表时间：
1992-05-15
影响因子：
7.4
通讯作者：
BRENNA, JT
中科院分区：
文献类型：
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作者：
GOODMAN, KJ;BRENNA, JT
使用高浓缩、均匀标记的脂肪酸（[U-C-13]），通过高精度气相色谱-燃烧同位素比质谱（GCC-IRMS）进行分析，已被评价为代谢示踪技术。C-13/C-12比率常规测定为对于> 10 ng脂肪酸精确度（SD）< 0.00001（delta（PDB）< 1份/千），对于30 pg脂肪酸样品精确度（SD）< 0.001（delta（PDB）< 100份/千），后者对应于100 fmol样品。显示人血浆组分中的基线脂肪酸C-13/C-12在10小时内波动不超过0.00004（Δ（PDB）4份/千份）。对一名78公斤的成年人给予10毫克剂量的42%C-13标记的硬脂酸后，在脂肪酸血浆部分中获得了大于0.001的C-13/C-12富集（δ（PDB）100份/千分之一）。在血浆中C-13/C-12富集范围小于0.0002（几乎等于13份/千份δ（PDB）单位）的范围内识别出生物动力学。提出了一种校正含碳衍生物引起的同位素比值污染的方法。高精度GCC-IRMS与高度富集的示踪剂一起使用，显示出与有机GC/MS相比具有稳定同位素示踪剂检测的优势，并且在剂量大小和分析效率方面上级放射性示踪剂方法。
The use of highly enriched, uniformly labeled fatty acid ([U-C-13]) with analysis by high-precision gas chromatography-combustion isotope ratio mass spectrometry (GCC-IRMS) has been evaluated as a metabolic tracer technique. C-13/C-12 ratios are routinely determined to precisions (SD) of < 0.00001 (delta(PDB) < 1 parts per thousand) for > 10 ng of fatty acid, and < 0.001 (delta(PDB) < 100 parts per thousand) for samples of 30 pg of fatty acid, the latter corresponding to a 100-fmol sample. Baseline fatty acid C-13/C-12 In human plasma fractions is shown 10 fluctuate not more than 0.00004 (delta(PDB) 4 parts per thousand) over 10 h. C-13/C-12 enrichments greater than 0.001 (delta(PDB) 100 parts per thousand) are obtained in a fatty acid plasma fraction subsequent to a 10-mg dose of 42% C-13-labeled stearic acid to a 78-kg adult. Blokinetics are discerned over an C-13/C-12 enrichment range of less than 0.0002 (almost-equal-to 13 parts per thousand in delta(PDB) units) in plasma. A means for correction of isotope ratio contamination due to carbon-containing derivatives is presented. High-precision GCC-IRMS used in concert with highly enriched tracers is shown to possess advantages versus organic GC/MS for stable isotopic tracer detection and is superior to radiotracer methods in terms of dose sizes and analysis efficiency.