Genetic dissection of a genomic region for a quantitative trait locus, Hd3, into two loci, Hd3a and Hd3b, controlling heading date in rice
Genetic dissection of a genomic region for a quantitative trait locus, Hd3, into two loci, Hd3a and Hd3b, controlling heading date in rice
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DOI：
10.1007/s00122-001-0813-0
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发表时间：
2002-04-01
影响因子：
5.4
通讯作者：
Yano, M
中科院分区：
文献类型：
--
作者：
Monna, L;Lin, HX;Yano, M
水稻光周期敏感基因HD3最初被定位于水稻6号染色体上，是一个与抽穗期相关的数量性状基因。利用早稻品种日本晴与籼稻品种Kasalath回交后代的大分离群体，对HD3进行了高分辨率连锁定位。为了确定HD3的基因分型，我们在自然条件下和短日照条件下进行了子代测试。结果在Hd3区发现了两个紧密连锁的基因座Hd3a和Hd3b。利用分子标记辅助选择技术从后代中筛选出Hd3a和Hd3b的近等基因系。Hd3a和Hd3b的遗传方式均为加性遗传。对Hd3a和Hd3b近等基因系的日长效应分析表明，Hd3a的Kasalath等位基因在短日照条件下促进抽穗，而Hd3b的Kasalath等位基因在长日照和自然田间条件下导致抽穗延迟。
The rice photoperiod sensitivity gene Hd3 was originally detected as a heading date-related quantitative trait locus localized on chromosome 6 of rice. High-resolution linkage mapping of Hd3 was performed using a large segregating population derived from advanced backcross progeny between a japonica variety, Nipponbare, and an indica variety, Kasalath. To determine the genotype of Hd3, we employed progeny testing under natural field and short-day conditions. As a result, two tightly linked loci, Hd3a and Hd3b, were identified in the Hd3 region. Nearly-isogenic lines for Hd3a and Hd3b were selected from progeny using marker-assisted selection. The inheritance mode of both Hd3a and Hd3b was found to be additive. Analysis of daylength response in nearly-isogenic lines of Hd3a and Hd3b showed that the Kasalath allele at Hd3a promotes heading under short-day conditions while that at Hd3b causes late heading under long-day and natural field conditions.