Beyond nanopore sizing: improving solid-state single-molecule sensing performance, lifetime, and analyte scope for omics by targeting surface chemistry during fabrication
Beyond nanopore sizing: improving solid-state single-molecule sensing performance, lifetime, and analyte scope for omics by targeting surface chemistry during fabrication
复制标题
DOI：
10.1088/1361-6528/ab8f4d
复制
发表时间：
2020-08-14
期刊：
影响因子：
3.5
通讯作者：
Kim, Min Jun
中科院分区：
文献类型：
--
作者：
D. Y. Bandara, Y. M. Nuwan;Saharia, Jugal;Kim, Min Jun
固态纳米孔（SSN）是单分子分辨率传感器，在实时生物聚合物分析中占有越来越大的份额，最突出的是DNA测序，但远非唯一。随着可控介电击穿（CDB）的出现，SSN的可及性有所增加，但仍然存在严重的基本挑战：开孔电流漂移和（不可逆的）分析物粘附。这些行为阻碍了商业应用的基础研究和设备开发，并且可能因不同分析物的化学复杂性和物理性质多样性而急剧加剧。我们展示了一个SSN制造方法注意到纳米孔表面化学在孔形成过程中，从而创建纳米孔氮化硅（SiNx）能够感测一个广泛的分析范围核酸（双链DNA），蛋白质（全人血清转铁蛋白）和聚糖（麦芽糖糊精）。与在没有这种综合方法的情况下制造的SiNx孔相反，孔在电解质中是欧姆的，在分析物移位（>1小时）期间在宽范围的孔直径（小于或类似于1小时）内具有极其稳定的开孔电流。
Solid-state nanopores (SSNs) are single-molecule resolution sensors with a growing footprint in real-time bio-polymer profiling-most prominently, but far from exclusively, DNA sequencing. SSNs accessibility has increased with the advent of controlled dielectric breakdown (CDB), but severe fundamental challenges remain: drifts in open-pore current and (irreversible) analyte sticking. These behaviors impede basic research and device development for commercial applications and can be dramatically exacerbated by the chemical complexity and physical property diversity of different analytes. We demonstrate a SSN fabrication approach attentive to nanopore surface chemistry during pore formation, and thus create nanopores in silicon nitride (SiNx) capable of sensing a wide analyte scope-nucleic acid (double-stranded DNA), protein (holo-human serum transferrin) and glycan (maltodextrin). In contrast to SiNx pores fabricated without this comprehensive approach, the pores are Ohmic in electrolyte, have extremely stable open-pore current during analyte translocation (>1 h) over a broad range of pore diameters (less than or similar to