Early gene expression profile of human skin to injury using high-density cDNA microarrays
Early gene expression profile of human skin to injury using high-density cDNA microarrays
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DOI：
10.1046/j.1524-475x.2001.00360.x
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发表时间：
2001-09-01
影响因子：
2.9
通讯作者：
Isik, F
中科院分区：
文献类型：
--
作者：
Cole, J;Tsou, R;Isik, F
正常伤口愈合中的干扰可以追溯到损伤后基因表达的扰动。为了解释皮肤损伤的正常和异常遗传反应，必须更好地定义未损伤皮肤和损伤皮肤的基线基因表达。本研究的目的是使用cDNA微阵列确定人皮肤损伤后即刻的基因表达谱。从5名接受乳房缩小手术的健康女性获得正常和损伤皮肤样品。在初始损伤后30分钟和1小时获得表皮和真皮的标本，提取RNA，逆转录成cDNA，并在4，000个基因的高密度cDNA微阵列膜上杂交。在30分钟时，损伤导致4，000个基因中的124个（3%）的基因表达的一致增加（> 2x）。这些基因主要参与转录和信号传导。在30分钟时，4，000个基因中没有一个减少（<2x）。000个基因的表达增加（1.15%），但4，000个基因中有264个（6.6%）降低超过2倍，表明许多结构基因沉默。我们已经确定了几个基因，即细胞因子信号转导抑制因子-1，rho HP 1和BB 1，它们在损伤后高度表达，并且可能在调节初始炎症反应中具有未被认识到的作用。这些数据提供了人类皮肤损伤后立即进行的基因表达的初步高通量分析，并显示了高通量分析在皮肤生物学和伤口修复中的实用性和未来的重要性。
Disturbances in normal wound healing may be traced to perturbations in gene expression following injury. To decipher normal and abnormal genetic responses to cutaneous injury, baseline gene expression of uninjured skin and injured skin must be better defined. Our aim for this study was to determine the gene expression profile of human skin immediately following injury using cDNA microarrays, Samples of normal and injured skin were obtained from 5 healthy females undergoing breast reduction surgery. Specimens of the epidermis and dermis were obtained at 30 minutes and 1 hour after the initial injury, RNA was extracted, reverse transcribed into cDNA and hybridized onto high-density cDNA microarray membranes of 4,000 genes. At 30 minutes, injury resulted in a consistent increase (> 2x) in gene expression of 124 out of 4,000 genes (3%), These genes were primarily involved in transcription and signaling, None of the 4,000 genes were decreased (< 2 x) at 30 minutes, At 1 hour only 46 out of the 4,000 genes were increased in expression (1.15%) but 264 out of 4,000 (6.6%) genes were decreased greater than 2 fold, indicating a silencing of many structural genes. We have identified several genes, namely, suppressor of cytokine signaling-1, rho HP1, and BB1, that are highly expressed after injury and may have an unappreciated role in regulating the initial inflammatory response. These data provide an initial high-throughput analysis of gene expression immediately following human skin injury and show the utility and future importance of high-throughput analysis in skin biology and wound repair.