Isoprene prevents the negative consequences of high temperature stress in Platanus orientalis leaves.
Isoprene prevents the negative consequences of high temperature stress in Platanus orientalis leaves.
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异戊二烯可防止悬铃木叶子中高温胁迫的负面后果。
DOI：
10.1071/fp06058
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
A. Edreva
中科院分区：
文献类型：
--
作者：
V. Velikova;F. Loreto;T. Tsonev;F. Brilli;A. Edreva
以1年生和2年生悬铃木为材料，研究了异戊二烯提高植物耐热性的现象。我们的目标是确定异戊二烯的排放是否取决于植物的年龄，以及不同的排放速度是否会影响不同年龄植物的耐热性。与一年生的植物相比，两年生的植物释放出更多的异戊二烯，并且具有更好的应对热应激的能力。经过高温(38℃，4 h)处理后，1年生和2年生植物释放异戊二烯的叶片的光合作用活性、过氧化氢含量、脂质过氧化和抗自由基活性均保持不变。然而，高温抑制了异戊二烯的光合作用和PSII效率，导致了H_2O_2的积累，提高了异戊二烯被磷霉素抑制的两个年龄段植物叶片的所有膜损伤指数和抗氧化能力。在热处理过程中用外源异戊二烯熏蒸异戊二烯抑制的叶片，减少了对光合作用能力的负面影响。这些结果进一步支持了异戊二烯在保护光合作用免受高温破坏方面发挥重要作用的观点。异戊二烯是植物抗热胁迫非酶防御的重要化合物，可能有助于清除活性氧(ROS)和膜稳定能力，特别是在发育成熟的植物中。
The phenomenon of enhanced plant thermotolerance by isoprene was studied in leaves of the same age of 1- or 2-year-old Platanus orientalis plants. Our goals were to determine whether the isoprene emission depends on the age of the plant, and whether different emission rates can influence heat resistance in plants of different age. Two-year-old plants emit greater amounts of isoprene and possess better capacity to cope with heat stress than 1-year-old plants. After a high temperature treatment (38°C for 4 h), photosynthetic activity, hydrogen peroxide content, lipid peroxidation and antiradical activity were preserved in isoprene emitting leaves of 1- and 2-year-old plants. However, heat inhibited photosynthesis and PSII efficiency, caused accumulation of H2O2, and increased all indices of membrane damage and antioxidant capacity in leaves of plants of both ages in which isoprene was inhibited by fosmidomycin. In isoprene-inhibited leaves fumigated with exogenous isoprene during the heat treatment, the negative effects on photosynthetic capacity were reduced. These results further support the notion that isoprene plays an important role in protecting photosynthesis against damage at high temperature. It is suggested that isoprene is an important compound of the non-enzymatic defence of plants against thermal stress, possibly contributing to scavenging of reactive oxygen species (ROS) and membrane stabilising capacity, especially in developed plants.