Proteomic Analysis of Haptoglobin and Amyloid A Protein Levels in Patients with Vivax Malaria
Proteomic Analysis of Haptoglobin and Amyloid A Protein Levels in Patients with Vivax Malaria
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DOI：
10.3347/kjp.2010.48.3.203
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发表时间：
2010-09-01
影响因子：
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通讯作者：
Kim, Tong-Soo
中科院分区：
文献类型：
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作者：
Bahk, Young Yil;Na, Byoung-Kuk;Kim, Tong-Soo
蛋白质组学领域的进步为开发针对人类疾病的诊断和治疗工具提供了巨大的机会。在这项研究中，我们分析了结合结合珠蛋白和淀粉样蛋白A蛋白的间日疟患者的丰富的血浆蛋白，2-DE，图像分析和质谱在正常的健康捐助者和间日疟患者之间的血浆中的耗尽。结果表明，在2-DE凝胶上，与健康供体相比，由于蛋白水解切割，间日疟患者血浆中触珠蛋白的表达水平显著降低或检测不到。间日疟患者血清淀粉样蛋白A含量明显升高，具有高度统计学意义。这两种蛋白是常见的急性期反应物，需要对大量患者进行进一步的大规模评价，以确定这些蛋白在间日疟患者中存在变化的可能临床意义。然而，我们的蛋白质组学分析表明，一些血浆蛋白，如触珠蛋白和血清淀粉样蛋白A，作为间日疟相关因子候选人的可行价值。
Advancements in the field of proteomics have provided great opportunities for the development of diagnostic and therapeutic tools against human diseases. In this study, we analyzed haptoglobin and amyloid A protein levels of vivax malaria patients with combinations of depletion of the abundant plasma proteins, 2-dimensional gel electrophoresis (2-DE), image analysis, and mass spectrometry in the plasma between normal healthy donors and vivax malaria patients. The results showed that the expression level of haptoglobin had become significantly lower or undetectable in the plasma of vivax malaria patients due to proteolytic cleavage when compared to healthy donors on 2-DE gels. Meanwhile, serum amyloid A protein was significantly increased in vivax malaria patient's plasma with high statistical values. These 2 proteins are common acute phase reactants and further large scale evaluation with a larger number of patient's will be necessary to establish the possible clinical meaning of the existential changes of these proteins in vivax malaria patients. However, our proteomic analysis suggests the feasible values of some plasma proteins, such as haptoglobin and serum amyloid A, as associating factor candidates for vivax malaria.