Comparative distribution of vasopressin V1b and oxytocin receptor messenger ribonucleic acids in brain
Comparative distribution of vasopressin V1b and oxytocin receptor messenger ribonucleic acids in brain
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DOI：
10.1210/en.139.12.5015
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发表时间：
1998-12-01
期刊：
影响因子：
4.8
通讯作者：
Ostrowski, NL
中科院分区：
文献类型：
--
作者：
Vaccari, C;Lolait, SJ;Ostrowski, NL
采用原位杂交组织化学方法，对雄性大鼠脑内抗利尿激素V1b受体（V1bR）和催产素受体（OTR）信使rna （mrna）的分布进行了比较。V1bR转录本存在于前脑和下丘脑，在中脑和后脑区域较少，与OTR转录本观察到的梯度相似。显微分析表明，表达V1bR的细胞具有典型的神经元形态，并证实了V1bR基因在嗅球、视上核、视交叉上核和下丘脑背内侧核、梨状皮质和内嗅皮质、海马、黑质和迷走神经背运动核等区域的表达。大部分表达V1bR mRNA的区域也表达OTR mRNA，尽管OTR基因的表达要比V1bR广泛得多。V1bR和OTR mRNA在脑内的分布不同，与Via受体mRNA的分布也不同。少数脑区仅表达V1bR转录本，如下丘脑背内侧核和嗅球外丛状层。其他脑区，如阿蒙角区、视交叉上核、黑质致密部和梨状皮质表达编码所有三种受体亚型（V1a、V1b和OTR）的mrna，而包括红核和视上核在内的脑区仅表达V1bR和OTR转录本。这些数据提示了脑内V1b、OTR和Via受体的功能特化。
The comparative distributions of the vasopressin V1b receptor (V1bR) and the oxytocin receptor (OTR) messenger RNAs (mRNAs) are described in male rat brain using in, situ hybridization histochemistry. V1bR transcripts were present in forebrain and hypothalamus and were less abundant in mid- and hindbrain regions, similar to the gradient observed with OTR transcripts. Microscopic analyses indicated that V1bR expressing cells typically demonstrated the morphology of neurons and confirmed V1bR gene expression in regions including the olfactory bulb, supraoptic, suprachiasmatic, and dorsomedial hypothalamic nuclei, piriform and entorhinal cortices, hippocampus, substantia nigra, and dorsal motor nucleus of the vagus. Most regions that expressed V1bR mRNA also expressed OTR mRNA, although OTR gene expression was much more extensive than that of the V1bR. V1bR and OTR mRNA distributions were distinct from each other and from that of the Via receptor mRNA in brain. A few brain regions express only V1bR transcripts such as the dorsomedial hypothalamic nucleus and the external plexiform layer of the olfactory bulb. Other brain regions, such as the fields of Ammon's horn, the suprachiasmatic nucleus, the substantia nigra pars compacta, and the piriform cortex express mRNAs that encode all three receptor subtypes (V1a, V1b, and OTR), whereas brain areas including the red nucleus and supraoptic nucleus express V1bR and OTR transcripts only. These data suggest functional specialization of the V1b, OTR and Via receptors in brain.