Myosin-based regulation of twitch and tetanic contractions in mammalian skeletal muscle.
Myosin-based regulation of twitch and tetanic contractions in mammalian skeletal muscle.
复制标题
DOI：
10.7554/elife.68211
复制
发表时间：
2021-06-14
期刊：
影响因子：
7.7
通讯作者：
Irving M
中科院分区：
文献类型：
--
作者：
Hill C;Brunello E;Fusi L;Ovejero JG;Irving M
时间分辨的X射线衍射分离的快速收缩肌肉的小鼠被用来显示肌球蛋白的粗丝的结构变化如何有助于肌肉收缩的调节，延长了以前的重点调节肌动蛋白的细丝。这项研究表明，肌肉激活涉及以下序列的结构变化：细丝激活，中断的螺旋阵列的肌球蛋白电机的特点，休息肌肉，肌球蛋白电机域的释放从折叠构象的细丝骨干，和肌动蛋白的附件。骨骼肌对单一动作电位刺激的“颤搐”反应中的生理力产生受到粗肌丝不完全激活和两种肌丝快速失活的限制。重复刺激后的肌肉松弛伴随着完全恢复的丝状骨干上的折叠电机构象，但不完全重组的螺旋阵列，揭示了在孤立的肌肉强直后增强的结构基础。
Time-resolved X-ray diffraction of isolated fast-twitch muscles of mice was used to show how structural changes in the myosin-containing thick filaments contribute to the regulation of muscle contraction, extending the previous focus on regulation by the actin-containing thin filaments. This study shows that muscle activation involves the following sequence of structural changes: thin filament activation, disruption of the helical array of myosin motors characteristic of resting muscle, release of myosin motor domains from the folded conformation on the filament backbone, and actin attachment. Physiological force generation in the ‘twitch’ response of skeletal muscle to single action potential stimulation is limited by incomplete activation of the thick filament and the rapid inactivation of both filaments. Muscle relaxation after repetitive stimulation is accompanied by a complete recovery of the folded motor conformation on the filament backbone but by incomplete reformation of the helical array, revealing a structural basis for post-tetanic potentiation in isolated muscles.
X射线衍射证据表明，在横纹肌肉中存在102.0- nm的横向周期性。
影响因子：
7.8
作者：
Bordas, J;Mant, G R;Diakun, G P;Nave, C
通讯作者：
Nave, C