In vitro reactivity of splenic lymphocytes from normal and UV-irradiated mice against syngeneic UV-induced tumors.
In vitro reactivity of splenic lymphocytes from normal and UV-irradiated mice against syngeneic UV-induced tumors.
复制标题
正常小鼠和紫外线照射小鼠的脾淋巴细胞对同基因紫外线诱导肿瘤的体外反应性。
DOI：
10.4049/jimmunol.118.4.1483
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发表时间：
1977
影响因子：
4.4
通讯作者：
M. Kripke
中科院分区：
文献类型：
--
作者：
G. Fortner;M. Kripke
慢性紫外线照射诱导的小鼠皮肤肿瘤具有高度的抗原性，并且在移植到正常的同基因受体后经常发生免疫排斥反应。在这项研究中，我们用体外微细胞毒性试验表征了这种免疫反应。单次注射紫外光诱导的纤维肉瘤细胞后，小鼠脾脏细胞有细胞毒活性。在去除粘附的脾脏细胞后，剩余的脾脏淋巴细胞对免疫肿瘤具有特异性细胞毒性，与其他同基因紫外线诱导或甲基胆蒽诱导的类似组织学类型的肿瘤无交叉反应性。与甲基胆蒽诱导的同基因肿瘤相比，细胞介导的对紫外线诱导肿瘤的反应性水平相当高，细胞毒性可归因于携带θ抗原的淋巴细胞群体。通过这种体外实验，我们比较了正常小鼠的反应，正常小鼠拒绝同基因肿瘤的攻击，与紫外线照射小鼠的反应，同基因紫外线诱导的肿瘤逐渐生长。肿瘤细胞接种后，未辐照（回归）小鼠的淋巴细胞显示出高度的细胞毒性，在注射后8天达到最高水平。相比之下，在肿瘤激发的紫外线照射（进展）小鼠的脾脏中未检测到反应性。
Skin tumors induced in mice by chronic ultraviolet (UV) irradiation are highly antigenic and are frequently immunologically rejected upon transplantation to normal syngeneic recipients. In this study we characterized this immune response with an in vitro microcytotoxicity test. Cytotoxic activity was present in the spleen cells of mice given a single injection of syngeneic UV-induced fibrosarcoma cells. After removal of adherent spleen cells, the remaining splenic lymphocytes were specifically cytotoxic for the immunizing tumor and showed no cross-reactivity with other syngeneic UV-induced or methylcholanthrene-induced tumors of similar histologic type. The level of cell-mediated reactivity against UV-induced tumors was quite high compared to that obtained with syngeneic tumors induced by methylcholanthrene, and the cytotoxicity was attributable to a population of theta antigen-bearing lymphocytes. With this in vitro test, we compared the response of normal mice, which reject a syngeneic tumor challenge, with that of UV-irradiated mice, in which the syngeneic UV-induced tumors grow progressively. After tumor cell inoculation, lymphocytes form the unirradiated (regressor) mice showed a high degree of cytotoxicity that reached a maximum level 8 days after injection. In contrast, no reactivity could be detected in the spleens of tumor-challenged UV-irradiated (progressor) mice.