Key Physiology of Anaerobic Ammonium Oxidation

Key Physiology of Anaerobic Ammonium Oxidation
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DOI:
10.1128/aem.65.7.3248-3250.1999
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发表时间:
1999-07
影响因子:
4.4
通讯作者:
M. Strous;J. Kuenen;M. Jetten
M. Strous;J. Kuenen;M. Jetten
中科院分区:
生物学2区
文献类型:
--
作者:
M. Strous;J. Kuenen;M. Jetten

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摘要对序批式反应器中厌氧氨氧化(anammox)聚集体的生长生理进行了定量研究。生理pH和温度范围分别为6.7至8.3和20至43°C。底物铵和亚硝酸盐的亲和常数均小于0.1毫克氮每升。厌氧氨氧化过程完全抑制亚硝酸盐浓度高于0.1克氮每升。添加痕量的任一种厌氧氨氧化中间体(每升肼1.4毫克氮或每升羟胺0.7毫克氮)完全恢复活性。
ABSTRACT The physiology of anaerobic ammonium oxidizing (anammox) aggregates grown in a sequencing batch reactor was investigated quantitatively. The physiological pH and temperature ranges were 6.7 to 8.3 and 20 to 43°C, respectively. The affinity constants for the substrates ammonium and nitrite were each less than 0.1 mg of nitrogen per liter. The anammox process was completely inhibited by nitrite concentrations higher than 0.1 g of nitrogen per liter. Addition of trace amounts of either of the anammox intermediates (1.4 mg of nitrogen per liter of hydrazine or 0.7 mg of nitrogen per liter of hydroxylamine) restored activity completely.