High expression of DDR1 is associated with the poor prognosis in Chinese patients with pancreatic ductal adenocarcinoma.
High expression of DDR1 is associated with the poor prognosis in Chinese patients with pancreatic ductal adenocarcinoma.
复制标题
DDR1高表达与中国胰腺导管腺癌患者不良预后相关
DOI：
10.1186/s13046-015-0202-1
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发表时间：
2015-08-22
期刊：
影响因子：
--
通讯作者：
Sun Y
中科院分区：
文献类型：
--
作者：
Huo Y;Yang M;Liu W;Yang J;Fu X;Liu D;Li J;Zhang J;Hua R;Sun Y
研究背景盘状结构域受体1（Discoidin domain receptors 1，DDR 1）是DDRs的一种亚型，是细胞形态发生、分化、迁移和侵袭的重要调控因子。在30例患者的队列中，我们通过实时定量PCR（RT-qPCR）或蛋白质印迹法检测了配对PDAC和相应相邻非肿瘤组织中的DDR 1表达。与正常邻近组织相比，PDAC中的DDR 1表达显著更高，证实了Oncomine数据库的结果。我们通过免疫组织化学方法验证了205例PDAC标本的非重叠队列中DDR 1表达。Kaplan-Meier生存曲线显示PDAC患者中DDR 1表达增加与预后不良相关（P= 0.013）。多因素考克斯回归分析显示，DDR 1表达、年龄、N分期和肝转移是PDAC的独立预后因素。
BackgroundDiscoidin domain receptors 1 (DDR1), a subtype of DDRs, has been reported as a critical modulator of cellular morphogenesis, differentiation, migration and invasion.Methods and resultsIn this study, we investigated the expression of DDR1 and its clinical association in Chinese patients with pancreatic ductal adenocarcinoma (PDAC). Across a cohort of 30 patients, we examined DDR1 expression in paired PDAC and corresponding adjacent non-tumor tissues by real-time quantitative PCR (RT-qPCR), or western blotting. DDR1 expression is significantly higher in PDAC, as compared to normal adjacent tissue, confirming results from the Oncomine databases. We validated DDR1 expression by immunohistochemistry across a non-overlapping cohort of 205 PDAC specimens. Kaplan-Meier survival curves indicate that increased expression of DDR1 is associated with a poor prognosis in PDAC patients (P= 0.013). Multivariate Cox regression analysis identified DDR1 expression, age, N classification and liver metastasis as independent prognostic factors in PDAC.ConclusionsThis study demonstrated that DDR1 can well serve as a novel prognostic biomarker in PDAC.