LAP, A NOVEL MEMBER OF THE C/EBP GENE FAMILY, ENCODES A LIVER-ENRICHED TRANSCRIPTIONAL ACTIVATOR PROTEIN
LAP, A NOVEL MEMBER OF THE C/EBP GENE FAMILY, ENCODES A LIVER-ENRICHED TRANSCRIPTIONAL ACTIVATOR PROTEIN
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DOI：
10.1101/gad.4.9.1541
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发表时间：
1990-09-01
影响因子：
10.5
通讯作者：
SCHIBLER, U
中科院分区：
文献类型：
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作者：
DESCOMBES, P;CHOJKIER, M;SCHIBLER, U
一个基因，编码肝脏富集的转录激活蛋白（cDNAs）已被分离。白蛋白是一种32 kD的蛋白质，在体内和体外刺激含有白蛋白D-启动子元件的嵌合基因的转录。EBP在其DNA结合和亮氨酸拉链结构域与C/EBP具有广泛的序列同源性（71%）。因此，这两种蛋白质显示出不可区分的DNA结合特异性，并且容易异源二聚化。另外，lap和cebp这两个基因都没有插入序列。虽然正确启动的转录本从cDNA 3基因积累在六个检查的组织。sbd。肝，肺，脾，肾，脑和睾丸。sbd。cDNA 3蛋白高度富集在肝细胞核。因此，在肝脏中的α蛋白的优先积累似乎是转录后调节。
A gene, encoding a liver-enriched transcriptional activator protein (LAP) has been isolated. LAP is a 32-kD protein that stimulates the transcription of chimeric genes containing albumin D-promoter elements both in vivo and in vitro. LAP shares extensive sequence homology (71%) in its DNA-binding and leucine zipper domains with C/EBP. As a consequence, these two proteins show an indistinguishable DNA-binding specificity and readily heterodimerize. In addition, both genes, lap and cebp, are devoid of intervening sequences. Although correctly initiated transcripts from the LAP gene accumulate in the six examined tissue.sbd.liver, lung, spleen, kidney, brain, and testis.sbd.LAP protein is highly enriched in liver nuclei. Thus, the preferential accumulation of LAP protein in liver appears to be regulated post-transcriptionally.