Extracts of Tsai Tai (Brassica chinensis): enhanced antioxidant activity and anti-aging effects both in vitro and in Caenorhabditis elegans
Extracts of Tsai Tai (Brassica chinensis): enhanced antioxidant activity and anti-aging effects both in vitro and in Caenorhabditis elegans
复制标题
菜苔（Brassica chinensis）提取物：在体外和秀丽隐杆线虫中增强抗氧化活性和抗衰老作用
DOI：
10.1039/c5fo01241d
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发表时间：
2016-01-01
期刊：
影响因子：
6.1
通讯作者：
Huang, Zebo
中科院分区：
文献类型：
--
作者：
Chen, Jing;Zhang, Ju;Huang, Zebo
蔡台因其美味的口感和营养价值，是亚洲国家消费最广泛的十字花科蔬菜之一。这项研究评估了3个蔡台(小白菜)品种的抗氧化能力和可能的相关健康益处，即鸿仔、白菜和彩心。通过清除DPPH自由基活性和还原能力实验评价提取物的体外活性。秀丽隐杆线虫被用作体内模型来评估有益健康的影响，包括抗氧化活性和延缓衰老。在体外，鸿仔提取物的抗氧化活性高于Pak Choi和Choi Sum，总酚含量与DPPH和RP值呈显著正相关。在体内，三种被测试的蔡泰提取物显著增强了对百草枯诱导的氧化应激的抵抗力，与对照组相比，存活率从15%提高到28%。然而，只有鸿仔提取物显著延长了秀丽线虫的寿命，与对照组相比，平均寿命增加了8%。通过DCF试验评估ROS的产生，获得了抗氧化保护的进一步证据。细胞内超氧化物歧化酶活性和丙二醛含量的分析证实了抗氧化保护作用的存在。这些结果表明，蔡台可能是天然抗氧化剂的良好来源，特别是鸿财台可以被开发为潜在的延缓衰老的膳食补充剂。
Tsai Tai is one of the most widely consumed Brassica vegetables in Asian countries because of its good taste and its nutritional benefits. This study evaluated the antioxidant capacity and possible associated health benefits of 3 Tsai Tai (Brassica chinensis) varieties, namely, Hon Tsai Tai, Pak Choi and Choi Sum. The DPPH radical scavenging ability and reducing power assays were performed to evaluate the in vitro activities of the extracts. Caenorhabditis elegans was used as an in vivo model for evaluation of beneficial health effects, including antioxidant activity and delayed aging. In vitro, the Hon Tsai Tai extract exhibited higher antioxidant activities than Pak Choi and Choi Sum, and the total phenolic contents were significantly correlated with the DPPH and RP values. In vivo, the three assayed Tsai Tai extracts significantly increased resistance against paraquat-induced oxidative stress with an increase in survival rates from 15% to 28% compared with controls. However, only the extract from Hon Tsai Tai significantly prolonged the lifespan of Caenorhabditis elegans, with an 8% increase in the mean lifespan with respect to controls. Further evidence of antioxidant protection was obtained by assessing ROS production via the DCF assay. The analyses of intracellular SOD activity and MDA content confirmed the existence of an antioxidant protective effect. These results suggest that Tsai Tai might serve as a good source of natural antioxidants, and in particular, Hon Tsai Tai could be explored as a potential dietary supplement to retard aging.