Increased Accumulation of Artemisinin and Anthocyanins in Artemisia annua Expressing the Arabidopsis Blue Light Receptor CRY1
Increased Accumulation of Artemisinin and Anthocyanins in Artemisia annua Expressing the Arabidopsis Blue Light Receptor CRY1
复制标题
表达拟南芥蓝光受体 CRY1 的青蒿中青蒿素和花青素的积累增加
DOI：
10.1007/s11105-008-0088-6
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发表时间：
2009-09-01
影响因子：
2.1
通讯作者：
Wang, Ling-Jian
中科院分区：
文献类型：
--
作者：
Hong, Gao-Jie;Hu, Wen-Li;Wang, Ling-Jian
青蒿素是从青蒿属植物中分离得到的一种倍半萜内酯，是一种治疗疟疾的有效药物。青蒿素在植物中的产量相对较低，受各种环境生长条件的影响。在拟南芥中，隐花色素1(CRY1)是感知光信号的关键受体之一，其过表达促进次生代谢物的积累。在本研究中，拟南芥CRY1的过表达导致青蒿素和花青素的积累增加。在蓝光下，高水平表达AtCRY1的转基因植株表现出各种蓝光诱导的反应，包括抑制下胚轴伸长和子叶伸长、矮化和气生器官的紫化。逆转录聚合酶链式反应分析表明，苯丙氨酸解氨酶和查尔酮合成酶编码基因的表达水平升高，花青素积累增加。对青蒿素生物合成中的3种重要酶--法尼基二磷酸合成酶(FPS)、紫穗槐-4，11-二烯合成酶(ADS)和CYP71AV1基因的表达分析表明，在AtCRY1植株中，FPS、ADS和CYP71AV1基因的转录丰度均增加。根据高效液相色谱分析，与对照相比，这些植物中的青蒿素含量增加了30%至40%。这些结果表明，光信号成分的改变可以为提高次生代谢物青蒿素的产量提供一条可行的途径。
Artemisinin, an effective drug against Plasmodium species that cause malaria, is a sesquiterpene lactone isolated from Artemisia annua. The yield of artemisinin in plants is relatively low, and it is influenced by various environmental growing conditions. In Arabidopsis, cryptochrome 1 (CRY1) is one of the key receptors that perceive light signals, and its overexpression promotes accumulation of secondary metabolites. In this study, overexpression of Arabidopsis CRY1 in A. annua resulted in increased accumulation of both artemisinin and anthocyanins. Under blue light, transgenic plants expressing high level of AtCRY1 showed a variety of blue light-induced responses, including inhibition of hypocotyl elongation and cotyledon expansion, dwarfism, and purpuring of aerial organs. Reverse transcription polymerase chain reaction analysis revealed that expression levels of the phenylalanine ammonia-lyase- and chalcone synthase-encoding genes were elevated and that anthocyanin accumulation was increased. Expression analysis of genes encoding farnesyl diphosphate synthase (FPS), amorpha-4,11-diene synthase (ADS), and CYP71AV1, three important enzymes in artemisinin biosynthesis, indicated that transcript abundance of all three genes, FPS, ADS, and CYP71AV1, increased in AtCRY1 plants. Based on high performance liquid chromatography analysis, artemisinin content in these plants increased by 30 similar to 40%, when compared to control. These results have demonstrated that alteration of light signaling components could provide a viable approach for enhancing production of the secondary metabolite artemisinin.