Interferon regulatory factors are transcriptional regulators of adipogenesis
Interferon regulatory factors are transcriptional regulators of adipogenesis
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DOI：
10.1016/j.cmet.2007.11.002
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发表时间：
2008-01-01
期刊：
影响因子：
29
通讯作者：
Rosen, Evan D.
中科院分区：
文献类型：
--
作者：
Eguchi, Jun;Yan, Qing-Wu;Rosen, Evan D.
我们试图通过实验和计算相结合的方法来确定脂肪形成中的转录途径。在这里，我们采用高通量DNA酶超敏性分析，以找到改变周围的关键脂肪细胞基因的染色质结构的区域。显示分化依赖性超敏反应变化的区域用于预测脂肪形成相关蛋白的结合位点。一个高得分的例子是干扰素调节因子（IRF）家族成员的结合基序。所有9种哺乳动物IRF mRNA的表达在脂肪形成过程中受到调节，并且有几种以分化依赖性方式与所鉴定的基序结合。此外，几种IRF蛋白抑制分化。这项分析表明，IRF蛋白在脂肪细胞生物学中的重要作用，并证明了这种方法在识别顺式和反式作用因子中的实用性，以前没有怀疑参与脂肪形成。
We have sought to identify transcriptional pathways in adipogenesis using an integrated experimental and computational approach. Here, we employ high-throughput DNase hypersensitivity analysis to find regions of altered chromatin structure surrounding key adipocyte genes. Regions that display differentiation-dependent changes in hypersensitivity were used to predict binding sites for proteins involved in adipogenesis. A high-scoring example was a binding motif for interferon regulatory factor (IRF) family members. Expression of all nine mammalian IRF mRNAs is regulated during adipogenesis, and several bind to the identified motifs in a differentiation-dependent manner. Furthermore, several IRF proteins repress differentiation. This analysis suggests an important role for IRF proteins in adipocyte biology and demonstrates the utility of this approach in identifying cis- and trans-acting factors not previously suspected to participate in adipogenesis.