Coregistration of diffuse optical spectroscopy and magnetic resonance imaging in a rat tumor model
Coregistration of diffuse optical spectroscopy and magnetic resonance imaging in a rat tumor model
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DOI：
10.1364/ao.42.002951
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发表时间：
2003-06-01
期刊：
影响因子：
1.9
通讯作者：
Nalcioglu, O
中科院分区：
文献类型：
--
作者：
Merritt, S;Bevilacqua, F;Nalcioglu, O
我们报告了近红外漫反射光谱（DOS）和磁共振成像（MRI）在动物模型肿瘤研究中的联合配准。使用组合的宽带稳态和频域装置来确定肿瘤中局部组织氧合血红蛋白、脱氧血红蛋白和水浓度。同时 MRI 共同配准提供了与光学测量相关的肿瘤结构（T-2 加权）和对比度增强图像。通过使用蒙特卡罗模拟，光学采样体积叠加在 MR 图像上，精确显示光学探测的组织结构。在 20 天内对 7 只大鼠进行了 DOS 和 MRI 配准测量，并将肿瘤组织分为三种类型：存活的、水肿的和坏死的。对每种组织类型进行光学测量的水浓度与总血红蛋白浓度的比率，显示水肿组织的值大于活组织（1.2+/-0.49M/μM对0.48+/-0.15M/μM）。组织血红蛋白氧饱和度 (StO(2)) 也显示组织类型之间存在很大差异：活组织的光学测量 StO(2) 值为 61 +/- 5%，而坏死组织测定的 StO(2) 值为 43 ± 6%。 (C) 2003 年美国光学学会。
We report coregistration of near-infrared diffuse optical spectroscopy (DOS) and magnetic resonance imaging (MRI) for the study of animal model tumors. A combined broadband steady-state and frequency-domain apparatus was used to determine tissue oxyhemoglobin, deoxyhemoglobin, and water concentration locally in tumors. Simultaneous MRI coregistration provided structural (T-2-weighted) and contrast-enhanced images of the tumor that were correlated with the optical measurements. By use of Monte Carlo simulations, the optically sampled volume was superimposed on the MR images, showing precisely which tissue structure was probed optically. DOS and MRI coregistration measurements were performed on seven rats over 20 days and were separated into three tumor tissue classifications: viable, edematous, and necrotic. A ratio of water concentration to total hemoglobin concentration, as measured optically, was performed for each tissue type and showed values for edematous tissue to be greater than viable tissue (1.2 +/- 0.49 M/ muM versus 0.48 +/- 0.15 M/ muM). Tissue hemoglobin oxygen saturation (StO(2)) also showed a large variation between tissue types: viable tissue had an optically measured StO(2) value of 61 +/- 5%, whereas StO(2) determined for necrotic tissue was 43 6%. (C) 2003 Optical Society of America.