Functionalization of PCL fibrous membrane with RGD peptide by a naturally occurring condensation reaction
Functionalization of PCL fibrous membrane with RGD peptide by a naturally occurring condensation reaction
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DOI：
10.1007/s11434-014-0336-0
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发表时间：
2014-04
影响因子：
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通讯作者：
Wenting Zheng;Di Guan;Yu Teng;Zhihong Wang;Suai Zhang;Lianyong Wang;D. Kong;Jun Zhang
中科院分区：
文献类型：
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作者：
Wenting Zheng;Di Guan;Yu Teng;Zhihong Wang;Suai Zhang;Lianyong Wang;D. Kong;Jun Zhang
聚己内酯(ε)是组织工程支架材料中广泛采用的材料。为了提高其细胞亲和力，本研究开发了一种新的方法，通过2-氰基苯并噻唑(CBT)与D-半胱氨酸的缩合反应在PCL表面引入具有生物活性的RGD多肽。采用静电纺丝法制备了PCL纤维膜，并用荧光显微镜、扫描电子显微镜(SEM)、X射线光电子能谱(XPS)和水接触角(WCA)对RGD功能化进行了表征。正如预期的那样，我们的结果表明RGD成功地固定在PCL表面。RGD改性提高了聚己内酯的亲水性，使其含水率从112.20°变为38.35°。3T3成纤维细胞的黏附、铺展和增殖能力也增强。因此，我们认为本研究中报道的方法是对疏水性PCL支架进行功能修饰的简便有效的方法。温和的反应条件也有利于生物活性分子在PCL上的共价固定化。
Poly(ε-caprolactone) (PCL) is widely adopted as an ingredient for tissue engineering scaffolds. To improve its cell affinity, in this study, we developed a new method to introduce bioactive RGD peptides onto the surface of PCL via condensation reaction between 2-cyanobenzothiazole (CBT) and D-cysteine. The PCL fibrous membranes were prepared by electrospinning, and RGD functionalization was characterized by fluorescence microscopy, scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and water contact angle (WCA). As expected, our results demonstrated the successful RGD immobilization on the surface of PCL. RGD modification improved the hydrophilicity of PCL, changing their WCA from 112.20° to 38.35°. Cell adhesion, spreading and proliferation of 3T3 fibroblasts were also enhanced. We therefore believe that the methods reported in this study was facile and effective for functional modification of the hydrophobic PCL scaffolds. The moderate reaction conditions are also suitable for covalent immobilization of bioactive molecules onto PCL.