Region-specific expression of mario reveals pivotal function of the anterior nondigit region on digit formation in chick wing bud
Region-specific expression of mario reveals pivotal function of the anterior nondigit region on digit formation in chick wing bud
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DOI：
10.1002/dvdy.20390
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发表时间：
2005-06-01
影响因子：
2.5
通讯作者：
Tamura, K
中科院分区：
文献类型：
--
作者：
Amano, T;Tamura, K
我们报道了一个新基因mario的区域特异性表达，该基因的表达域位于发育中的鸡翅芽中推测和发育中的第2指区域的远端。mario的前部区域特异性表达与第2指的存在对应良好，并且命运图分析表明，mario在早期阶段的表达代表假定的第2指区域。使用mario表达作为第2趾区域的区域特异性标记，进行了几次外科手术以了解鸡翅膀中的第2趾发育。伴随着极化活性区（ZPA）植入的细胞命运追踪显示，在ZPA植入到翅芽的前部或中部区域中的额外的第2指是从原始的第2指区域（mario阳性区域）衍生的。手术操作显示，前非手指区域有抑制作用的数字2的形成。综上所述，这些结果表明，最前面的区域，包括前坏死区，限制了第2指区域的位置，限制了第2指区域的前缘，并防止其扩展。(c)2005 Wiley-Liss，Inc.
We report the region-specific expression of a novel gene, named mario, whose expression domain is in the distal tip of the presumptive and developing digit 2 region in the developing chick wing bud. The anterior region-specific expression of mario corresponds well with the presence of digit 2, and fate map analysis showed that mario expression at early stages represents the presumptive digit 2 region. Using mario expression as a region-specific marker for the digit 2 region, several surgical operations were performed to obtain insights into digit 2 development in the chick wing. Cell fate tracing concomitant with a zone of polarizing activity (ZPA) implantation revealed that an additional digit 2 in the ZPA implantation into the anterior or middle region of wing bud is derived from the original digit 2 region (mario-positive region). Surgical manipulations revealed that the anterior nondigit region has an inhibitory effect on digit 2 formation. Taken together, these results suggest that the most-anterior region, including the anterior necrotic zone, restricts the position of digit 2 region by limiting the anterior border of the digit 2 region and preventing its expansion. (c) 2005 Wiley-Liss, Inc.