Further analysis of presence of peptides in dopamine neurons. Cholecystokinin, peptide histidine-isoleucine/vasoactive intestinal polypeptide and substance P in rat supramammillary region and mesencephalon.
Further analysis of presence of peptides in dopamine neurons. Cholecystokinin, peptide histidine-isoleucine/vasoactive intestinal polypeptide and substance P in rat supramammillary region and mesencephalon.
复制标题
进一步分析多巴胺神经元中肽的存在。
DOI：
10.1007/bf00250598
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发表时间：
1988
影响因子：
2
通讯作者：
Goldstein,M
中科院分区：
文献类型：
--
作者：
Seroogy,K;Tsuruo,Y;Hökfelt,T;Walsh,J;Fahrenkrug,J;Emson,PC;Goldstein,M
采用双标记结合洗脱-免疫荧光技术分析大鼠乳上区和中脑神经元中缩胆素（CCK）、组氨酸异亮氨酸(PHI)/血管活性肠多肽（VIP）、P物质和儿茶酚胺合成酶酪氨酸羟化酶的共存程度。约50%的含PHI/ vip的核周和约25%的乳糜上区cck阳性细胞体表现出两种肽的共存。在这些双标记的神经元中，只有一小部分被发现含有酪氨酸羟化酶的免疫染色（表明这些细胞中含有多巴胺）。低比例的神经元含有这种酶加上CCK-或PHI/ vip样免疫反应性。该区域酪氨酸羟化酶阳性神经元具有p样物质免疫反应性的比例较低，反之亦然。在其他区域，少量脑室周围和导尿管周围区域的神经元被CCK、PHI/VIP和酪氨酸羟化酶免疫染色。在腹侧被盖区很少观察到单例三标记（CCK-PHI/VIP-TH）体。这些数据进一步证明了多肽/多肽和多肽/单胺在中枢神经系统中共存。CCK-PHI/VIP共定位（可能包括少量多巴胺能成分）以及P物质和酪氨酸羟化酶在乳突上区神经元内共存，并广泛投射到前脑的许多区域，这表明这些神经肽可能共存于这些通路中的某些通路中，并可能在大脑的几个不同区域共同释放。
Double-labeling combined with elution-restaining immunofluorescence techniques were used to analyze the extent of coexistence among the peptides cholecystokinin (CCK), peptide histidine-isoleucine (PHI)/vasoactive intestinal polypeptide (VIP), substance P and the catecholamine-synthesizing enzyme tyrosine hydroxylase in neurons of the supramammillary region and mesencephalon of the rat. Approximately 50% of the PHI/VIP-containing perikarya and about 25% of the CCK-positive cell bodies in the supramammillary region exhibited coexistence of both peptides. Only a very minor portion of these double-labeled neurons were also found to contain immunostaining for tyrosine hydroxylase (indicative of dopamine in these cells). A low percentage of the neurons contained the enzyme plus either CCK- or PHI/VIP-like immunoreactivity. A low proportion of the tyrosine hydroxylase-positive neurons in this region contained substance P-like immunoreactivity and vice versa. In other areas, small numbers of neurons in periventricular and periaqueductal regions were found to be immuno-stained for CCK, PHI/VIP and tyrosine hydroxylase. Single examples of triple-labeled (CCK-PHI/VIP-TH) somata were infrequently observed in the ventral tegmental area. These data provide further evidence of peptide/peptide and peptide/monoamine coexistence in the central nervous system. The demonstration of CCK-PHI/VIP colocalization (possibly including a minor dopaminergic component) and of substance P and tyrosine hydroxylase coexistence within neurons of the supramammillary region, which has widespread projections to many areas of the forebrain, suggests that these neuropeptides may coexist in some of these pathways and perhaps be co-released in several different regions of the brain.