Murine models of Chlamydia pneumoniae infection and atherosclerosis
Murine models of Chlamydia pneumoniae infection and atherosclerosis
复制标题
DOI：
10.1086/513986
复制
发表时间：
1997-04-01
影响因子：
6.4
通讯作者：
Campbell, LA
中科院分区：
文献类型：
--
作者：
Moazed, TC;Kuo, CC;Campbell, LA
肺炎衣原体已被证实存在于动脉粥样硬化病变中，但不存在于正常动脉中。需要一种肺炎衣原体和动脉粥样硬化的动物模型来研究该生物体在动脉粥样硬化中的作用。评价了自发发展动脉粥样硬化的载脂蛋白（apo）E缺陷转基因小鼠和仅在致动脉粥样硬化饮食中发展动脉粥样硬化的C57 BL/6 J小鼠。在apoE基因缺陷转基因小鼠的单次和多次鼻内接种后，C。接种后20周，25%~ 100%的小鼠在肺、主动脉和脾中检出肺炎克雷伯氏菌。在动脉粥样硬化病变内检测到肺炎。在接受非致动脉粥样硬化饮食的C57 BL/6 J小鼠中，C.肺炎克雷伯氏菌经鼻接种2周后，8%的小鼠主动脉中检测到肺炎克雷伯氏菌。pneumoniae的嗜性提示C. pneumoniae肺炎to the lesion病变.这些小鼠模型对研究C.肺炎在动脉粥样硬化中的作用
Chlamydia pneumoniae have been demonstrated in atherosclerotic lesions but not in normal arteries. An animal model of both C, pneumoniae and atherosclerosis is needed to investigate the role of the organism in atherosclerosis. Apolipoprotein (apo) E-deficient transgenic mice, which spontaneously develop atherosclerosis, and C57BL/6J mice, which only develop atherosclerosis on an atherogenic diet, were evaluated. Following single and multiple intranasal inoculations of apoE-deficient transgenic mice, C. pneumoniae were detected in lung, aorta, and spleen for 20 weeks after inoculation in 25%-100% of mice, In the aorta, C. pneumoniae were detected within the atherosclerotic lesion. In C57BL/6J mice on a nonatherogenic diet, C. pneumoniae were detected in the aorta only 2 weeks after a single intranasal inoculation in 8% of mice, The persistence of C. pneumoniae in atheromas suggests a tropism of C. pneumoniae to the lesion. These mouse models should be useful for studying the pathogenic role of C. pneumoniae in atherosclerosis.