A reevaluation of the roles of the O2-dependent and O2-independent microbicidal systems of phagocytes.
A reevaluation of the roles of the O2-dependent and O2-independent microbicidal systems of phagocytes.
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重新评估吞噬细胞 O2 依赖性和 O2 非依赖性杀菌系统的作用。
DOI：
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
J. Weiss
中科院分区：
文献类型：
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作者：
P. Elsbach;J. Weiss
近年来，吞噬细胞特别是中性粒细胞的杀菌和细胞毒性作用主要归因于依赖于对细菌有毒的化学反应性衍生物的积累的O2依赖性系统。这一观点已被重新审视的光O2独立的杀菌蛋白，已从中性粒细胞纯化的性能和效力的新观察。这些蛋白质包括几种酶以及一种颗粒相关蛋白，其特异性地作用于某些革兰氏阴性细菌。易感细菌暴露于后一种蛋白质会产生三种影响：繁殖能力丧失，包膜外膜渗透性离散增加，以及作用于磷脂和肽聚糖的降解酶在细菌包膜中活化。它的结论是，有效的抗菌活性取决于O2-独立的杀菌蛋白，是高度特定的某些微生物物种和O2-需要系统，非特异性攻击所有细胞的共存。
In recent years the bactericidal and cytotoxic actions of phagocytes and particularly neutrophils have been attributed mainly to O2-dependent systems that depend on the accumulation of chemically reactive derivatives toxic to the bacteria. This view has been reexamined in the light of new observations on the properties and potency of O2-independent bactericidal proteins that have been purified from neutrophils. These proteins include several enzymes as well as a granule-associated protein that acts specifically against certain gram-negative bacteria. Exposure of susceptible bacteria to this latter protein produces three effects: loss of ability to multiply, a discrete increase in permeability of the outer membrane of the envelope, and activation in the bacterial envelope of degradative enzymes that act on phospholipids and peptidoglycan. It is concluded that effective antimicrobial activity rests on the coexistence of O2-independent bactericidal proteins that are highly specific for certain microbial species and O2-requiring systems that nonspecifically attack all cells.