Efficient embryonic culture method for the Japanese striped snake, Elaphe quadrivirgata, and its early developmental stages
Efficient embryonic culture method for the Japanese striped snake, Elaphe quadrivirgata, and its early developmental stages
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日本条纹蛇Elaphequadrivirgata及其早期发育阶段的高效胚胎培养方法
DOI：
10.1111/dgd.12157
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发表时间：
2014
影响因子：
2.5
通讯作者：
A. Kuroiwa and T. Suzuki
中科院分区：
文献类型：
--
作者：
Y. Matsubara;A. Sakai;A. Kuroiwa and T. Suzuki
蛇类胚胎的形态发生是发育生物学家的一个难以捉摸而又迷人的研究目标。然而，由于怀孕蛇的可用性有限，以及需要在不知道受精日期的情况下直接从怀孕蛇产卵前收获早期胚胎，因此很少有关于早期蛇胚胎发育的数据。以四条锦蛇（Elaphe quadrivirgata）为材料，建立了蛇早期胚胎体外培养方法。这种方法，我们命名为“香肠式（SS）培养”，允许我们在每个实验的特定阶段收获蛇胚胎。利用该培养系统，我们计算了产卵前早期的体节形成率。6/7 ~ 12/13体节期的平均体节形成速率为145.9min，60/70 ~ 80/91体节期的平均体节形成速率为42.4min，113-115体节期和126/127体节期的平均体节形成速率为71 min。因此，体节的形成率，我们观察到在早期蛇胚胎发生随时间而变化。我们还描述了一个发展分期系列forE. quadrivirgata。这是第一次通过高分辨率全色图像报告产卵前早期蛇胚胎发生的发育系列。我们认为SS培养系统是一种简单易行的蛇早期胚胎性行为的活体处理方法。
The morphogenesis of snake embryos is an elusive yet fascinating research target for developmental biologists. However, few data exist on development of early snake embryo due to limited availability of pregnant snakes, and the need to harvest early stage embryos directly from pregnant snakes before oviposition without knowing the date of fertilization. We established anex vivoculture method for early snake embryos using the Japanese striped snake,Elaphe quadrivirgata. This method, which we named “sausage‐style (SS) culture”, allows us to harvest snake embryos at specific stages for each experiment. Using this SS culture system, we calculated somite formation rate at early stages before oviposition. The average somite formation rate between 6/7 and 12/13 somite stages was 145.9 min, between 60/70 and 80/91 somite stages 42.4 min, and between 113–115 and 126/127 somite stages 71 min. Thus, somite formation rate that we observed during early snake embryogenesis was changed over time. We also describe a developmental staging series forE. quadrivirgata. This is the first report of a developmental series of early snake embryogenesis prior to oviposition by full‐color images with high‐resolution. We propose that the SS culture system is an easy method for treating early snake embryosex vivo.