Response of cancer cells to molecular interruption of the CK2 signal
Response of cancer cells to molecular interruption of the CK2 signal
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DOI：
10.1023/a:1013112908734
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发表时间：
2001-01-01
影响因子：
4.3
通讯作者：
Ahmed, K
中科院分区：
文献类型：
--
作者：
Wang, HM;Davis, A;Ahmed, K
蛋白激酶CK2是细胞存活、生长和增殖的关键细胞信号之一。人们观察到，在研究过的各种癌症中，它都会升高。各种观察表明，CK2的适度失调可能会深刻地影响细胞反应。我们研究了通过使用反义寡脱氧核苷酸（ODN）对CK2的α和β亚基干扰CK2信号在各种癌细胞系中的作用。我们的研究结果表明，反义ck2 - α和反义ck2 - β odn以剂量和时间依赖的方式显著影响这些癌细胞的细胞活力。在大多数测试的细胞中，反义ck2 - α比反义ck2 - β更有效。反义ODN的作用似乎随细胞类型的不同而不同；然而，在所有情况下，观察到细胞凋亡的强烈诱导。值得注意的是，反义ODN对核基质中CK2活性的影响相对较小，而对细胞凋亡的诱导作用要强得多。这表明CK2的适度下调可以在癌细胞中引起更大的凋亡反应。
Protein kinase CK2 is one of the key cellular signals for cell survival, growth, and proliferation. It is has been observed to be elevated in various cancers that have been examined. Various observations suggest that moderate dysregulation of CK2 may profoundly influence the cell response. We have examined the effects of interfering with the CK2 signal in various cancer cell lines by employing antisense oligodeoxynucleotides (ODN) against the alpha and beta subunits of CK2. Our results demonstrate that antisense CK2-alpha and antisense CK2-beta ODNs markedly influence cell viability of these cancer cells in a dose and time-dependent manner. Antisense CK2-alpha was slightly more effective than antisense CK2-beta in most of the cells tested. The efficacy of the antisense ODN seemed to vary with the cell type; however, in all cases potent induction of apoptosis was observed. Significantly, the effects of the antisense ODN on the CK2 activity in the nuclear matrix were relatively small compared to the much stronger induction of apoptosis in cells. This suggests that modest down-regulation of CK2 can evoke a much greater apoptotic response in cancer cells.